Cargando…
Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures
In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal ce...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098861/ https://www.ncbi.nlm.nih.gov/pubmed/30154866 http://dx.doi.org/10.1155/2018/8631432 |
_version_ | 1783348546324398080 |
---|---|
author | Liu, Jun Higgins, Claire A. Whitehouse, Jenna C. Harris, Susan J. Crawford, Heather Christiano, Angela M. Lako, Majlinda Hole, Nicholas Jahoda, Colin A. B. |
author_facet | Liu, Jun Higgins, Claire A. Whitehouse, Jenna C. Harris, Susan J. Crawford, Heather Christiano, Angela M. Lako, Majlinda Hole, Nicholas Jahoda, Colin A. B. |
author_sort | Liu, Jun |
collection | PubMed |
description | In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within follicles in situ in a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications. |
format | Online Article Text |
id | pubmed-6098861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60988612018-08-28 Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures Liu, Jun Higgins, Claire A. Whitehouse, Jenna C. Harris, Susan J. Crawford, Heather Christiano, Angela M. Lako, Majlinda Hole, Nicholas Jahoda, Colin A. B. Stem Cells Int Research Article In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within follicles in situ in a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications. Hindawi 2018-08-02 /pmc/articles/PMC6098861/ /pubmed/30154866 http://dx.doi.org/10.1155/2018/8631432 Text en Copyright © 2018 Jun Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Jun Higgins, Claire A. Whitehouse, Jenna C. Harris, Susan J. Crawford, Heather Christiano, Angela M. Lako, Majlinda Hole, Nicholas Jahoda, Colin A. B. Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title | Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title_full | Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title_fullStr | Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title_full_unstemmed | Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title_short | Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures |
title_sort | hair follicle dermal cells support expansion of murine and human embryonic and induced pluripotent stem cells and promote haematopoiesis in mouse cultures |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098861/ https://www.ncbi.nlm.nih.gov/pubmed/30154866 http://dx.doi.org/10.1155/2018/8631432 |
work_keys_str_mv | AT liujun hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT higginsclairea hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT whitehousejennac hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT harrissusanj hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT crawfordheather hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT christianoangelam hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT lakomajlinda hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT holenicholas hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures AT jahodacolinab hairfollicledermalcellssupportexpansionofmurineandhumanembryonicandinducedpluripotentstemcellsandpromotehaematopoiesisinmousecultures |