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Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method
A quick and cost-effective method to analyze leafy vegetable nitrate on spectrometry was developed and compared with a standard method using high performance liquid chromatography (HPLC). This method was designed to use ion-exchange solid phase extraction (SPE) cartridges in reducing interference fr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098929/ https://www.ncbi.nlm.nih.gov/pubmed/30174694 http://dx.doi.org/10.1155/2018/6285867 |
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author | Yu, Tzu-Hsien Hsieh, Shuo-Ping Su, Chien-Ming Huang, Feng-Jung Hung, Chien-Che Yiin, Lih-Ming |
author_facet | Yu, Tzu-Hsien Hsieh, Shuo-Ping Su, Chien-Ming Huang, Feng-Jung Hung, Chien-Che Yiin, Lih-Ming |
author_sort | Yu, Tzu-Hsien |
collection | PubMed |
description | A quick and cost-effective method to analyze leafy vegetable nitrate on spectrometry was developed and compared with a standard method using high performance liquid chromatography (HPLC). This method was designed to use ion-exchange solid phase extraction (SPE) cartridges in reducing interference from organic matrices to meet the criterion of an existing method for analyzing nitrate in wastewater. Nine vegetables (bok coy, cabbage, celery, Chinese cabbage, Chinese kale, lettuce, mustard green, pak choi, and water spinach) were selected for the method testing with three replicates being conducted for each vegetable. The nitrate contents ranged from 800 to 4,300 μg/g, with bok coy, celery, and pak choi being the highest. Data derived from spectrometry and HPLC were close to each other with most relative errors being within ±10% and were highly correlated with an R square value of 0.969. Stability testing and spike analysis resulted in a mean coefficient of variation lower than 6% and a mean recovery rate of 83.7%, suggesting reliability of the method. In addition, both the cost and time consumption for using this method were lower than the standard method using HPLC or ion chromatography, making this spectrometric method a good alternative for analysis of leafy vegetable nitrate. |
format | Online Article Text |
id | pubmed-6098929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60989292018-09-02 Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method Yu, Tzu-Hsien Hsieh, Shuo-Ping Su, Chien-Ming Huang, Feng-Jung Hung, Chien-Che Yiin, Lih-Ming Int J Anal Chem Research Article A quick and cost-effective method to analyze leafy vegetable nitrate on spectrometry was developed and compared with a standard method using high performance liquid chromatography (HPLC). This method was designed to use ion-exchange solid phase extraction (SPE) cartridges in reducing interference from organic matrices to meet the criterion of an existing method for analyzing nitrate in wastewater. Nine vegetables (bok coy, cabbage, celery, Chinese cabbage, Chinese kale, lettuce, mustard green, pak choi, and water spinach) were selected for the method testing with three replicates being conducted for each vegetable. The nitrate contents ranged from 800 to 4,300 μg/g, with bok coy, celery, and pak choi being the highest. Data derived from spectrometry and HPLC were close to each other with most relative errors being within ±10% and were highly correlated with an R square value of 0.969. Stability testing and spike analysis resulted in a mean coefficient of variation lower than 6% and a mean recovery rate of 83.7%, suggesting reliability of the method. In addition, both the cost and time consumption for using this method were lower than the standard method using HPLC or ion chromatography, making this spectrometric method a good alternative for analysis of leafy vegetable nitrate. Hindawi 2018-08-05 /pmc/articles/PMC6098929/ /pubmed/30174694 http://dx.doi.org/10.1155/2018/6285867 Text en Copyright © 2018 Tzu-Hsien Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yu, Tzu-Hsien Hsieh, Shuo-Ping Su, Chien-Ming Huang, Feng-Jung Hung, Chien-Che Yiin, Lih-Ming Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title | Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title_full | Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title_fullStr | Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title_full_unstemmed | Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title_short | Analysis of Leafy Vegetable Nitrate Using a Modified Spectrometric Method |
title_sort | analysis of leafy vegetable nitrate using a modified spectrometric method |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098929/ https://www.ncbi.nlm.nih.gov/pubmed/30174694 http://dx.doi.org/10.1155/2018/6285867 |
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