Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2

Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed ChIP-Seq of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 bindin...

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Autores principales: Ito-Ishida, Aya, Yamalanchili, Hari K., Shao, Yingyao, Baker, Steven A., Heckman, Laura D., Lavery, Laura A., Kim, Ji-yoen, Lombardi, Laura M., Sun, Yaling, Liu, Zhandong, Zoghbi, Huda Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099063/
https://www.ncbi.nlm.nih.gov/pubmed/29802390
http://dx.doi.org/10.1038/s41593-018-0155-8
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author Ito-Ishida, Aya
Yamalanchili, Hari K.
Shao, Yingyao
Baker, Steven A.
Heckman, Laura D.
Lavery, Laura A.
Kim, Ji-yoen
Lombardi, Laura M.
Sun, Yaling
Liu, Zhandong
Zoghbi, Huda Y.
author_facet Ito-Ishida, Aya
Yamalanchili, Hari K.
Shao, Yingyao
Baker, Steven A.
Heckman, Laura D.
Lavery, Laura A.
Kim, Ji-yoen
Lombardi, Laura M.
Sun, Yaling
Liu, Zhandong
Zoghbi, Huda Y.
author_sort Ito-Ishida, Aya
collection PubMed
description Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed ChIP-Seq of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.
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spelling pubmed-60990632018-11-25 Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2 Ito-Ishida, Aya Yamalanchili, Hari K. Shao, Yingyao Baker, Steven A. Heckman, Laura D. Lavery, Laura A. Kim, Ji-yoen Lombardi, Laura M. Sun, Yaling Liu, Zhandong Zoghbi, Huda Y. Nat Neurosci Article Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed ChIP-Seq of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent. 2018-05-25 2018-06 /pmc/articles/PMC6099063/ /pubmed/29802390 http://dx.doi.org/10.1038/s41593-018-0155-8 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ito-Ishida, Aya
Yamalanchili, Hari K.
Shao, Yingyao
Baker, Steven A.
Heckman, Laura D.
Lavery, Laura A.
Kim, Ji-yoen
Lombardi, Laura M.
Sun, Yaling
Liu, Zhandong
Zoghbi, Huda Y.
Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title_full Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title_fullStr Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title_full_unstemmed Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title_short Genome Wide Distribution of Linker Histone H1.0 is Independent of MeCP2
title_sort genome wide distribution of linker histone h1.0 is independent of mecp2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099063/
https://www.ncbi.nlm.nih.gov/pubmed/29802390
http://dx.doi.org/10.1038/s41593-018-0155-8
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