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Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects

OBJECTIVES: To investigate whether mice develop tolerance to the anxiolytic‐like and anticonvulsant effects of subchronic treatment with EA (the styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction of Polygala sabulosa), as well as any withdrawal symptoms after abrupt discontinuation; to compa...

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Autores principales: Duarte, Filipe Silveira, Duzzioni, Marcelo, Prim, Rafael Luiz, Cardozo, Alcíbia Maia, dos Santos, Claudia Regina, da Silva, Maria Goretti, Shiozawa, Maria Beatriz Cacese, Mendes, Beatriz Garcia, Tizziani, Tiago, Brighente, Inês Maria Costa, Pizzolatti, Moacir Geraldo, de Lima, Thereza Christina Monteiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099234/
https://www.ncbi.nlm.nih.gov/pubmed/29956326
http://dx.doi.org/10.1111/jphp.12960
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author Duarte, Filipe Silveira
Duzzioni, Marcelo
Prim, Rafael Luiz
Cardozo, Alcíbia Maia
dos Santos, Claudia Regina
da Silva, Maria Goretti
Shiozawa, Maria Beatriz Cacese
Mendes, Beatriz Garcia
Tizziani, Tiago
Brighente, Inês Maria Costa
Pizzolatti, Moacir Geraldo
de Lima, Thereza Christina Monteiro
author_facet Duarte, Filipe Silveira
Duzzioni, Marcelo
Prim, Rafael Luiz
Cardozo, Alcíbia Maia
dos Santos, Claudia Regina
da Silva, Maria Goretti
Shiozawa, Maria Beatriz Cacese
Mendes, Beatriz Garcia
Tizziani, Tiago
Brighente, Inês Maria Costa
Pizzolatti, Moacir Geraldo
de Lima, Thereza Christina Monteiro
author_sort Duarte, Filipe Silveira
collection PubMed
description OBJECTIVES: To investigate whether mice develop tolerance to the anxiolytic‐like and anticonvulsant effects of subchronic treatment with EA (the styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction of Polygala sabulosa), as well as any withdrawal symptoms after abrupt discontinuation; to compare the effects of EA with those of diazepam (DZP) on withdrawal‐induced anxiety; and to evaluate the toxicity of EA according to OECD guidelines. METHODS: Male or female mice were acutely or subchronically treated with EA or DZP, and their tolerance to anxiolytic (evaluated in the elevated plus maze, EPM) and anticonvulsant effects (measured against pentylenetetrazole (PTZ)‐induced convulsions) were investigated. Other groups received EA or DZP for 28 days followed by withdrawal, being the anxiety‐like behaviour evaluated in the EPM. KEY FINDINGS: Both acute and subchronic treatments with EA induced an anxiolytic effect in the EPM. The anticonvulsant activity of DZP, but not EA, was reduced by protracted treatment. EA withdrawal retained the anxiolytic profile, while DZP withdrawal induced anxiogenesis. EA counteracted the anxiogenic‐like actions of DZP withdrawal. EA has low toxicity as it did not cause any changes in the biochemical, haematological and histopathological markers. CONCLUSIONS: EA avoids the development of tolerance to its anxiolytic‐like and anticonvulsant actions, and does not promote withdrawal syndrome. EA does not cause relevant toxic effects in rodents.
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spelling pubmed-60992342018-08-23 Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects Duarte, Filipe Silveira Duzzioni, Marcelo Prim, Rafael Luiz Cardozo, Alcíbia Maia dos Santos, Claudia Regina da Silva, Maria Goretti Shiozawa, Maria Beatriz Cacese Mendes, Beatriz Garcia Tizziani, Tiago Brighente, Inês Maria Costa Pizzolatti, Moacir Geraldo de Lima, Thereza Christina Monteiro J Pharm Pharmacol Drugs from Natural Sources OBJECTIVES: To investigate whether mice develop tolerance to the anxiolytic‐like and anticonvulsant effects of subchronic treatment with EA (the styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction of Polygala sabulosa), as well as any withdrawal symptoms after abrupt discontinuation; to compare the effects of EA with those of diazepam (DZP) on withdrawal‐induced anxiety; and to evaluate the toxicity of EA according to OECD guidelines. METHODS: Male or female mice were acutely or subchronically treated with EA or DZP, and their tolerance to anxiolytic (evaluated in the elevated plus maze, EPM) and anticonvulsant effects (measured against pentylenetetrazole (PTZ)‐induced convulsions) were investigated. Other groups received EA or DZP for 28 days followed by withdrawal, being the anxiety‐like behaviour evaluated in the EPM. KEY FINDINGS: Both acute and subchronic treatments with EA induced an anxiolytic effect in the EPM. The anticonvulsant activity of DZP, but not EA, was reduced by protracted treatment. EA withdrawal retained the anxiolytic profile, while DZP withdrawal induced anxiogenesis. EA counteracted the anxiogenic‐like actions of DZP withdrawal. EA has low toxicity as it did not cause any changes in the biochemical, haematological and histopathological markers. CONCLUSIONS: EA avoids the development of tolerance to its anxiolytic‐like and anticonvulsant actions, and does not promote withdrawal syndrome. EA does not cause relevant toxic effects in rodents. John Wiley and Sons Inc. 2018-06-28 2018-09 /pmc/articles/PMC6099234/ /pubmed/29956326 http://dx.doi.org/10.1111/jphp.12960 Text en © 2018 The Authors. Journal of Pharmacy and Pharmacology published by John Wiley & Sons Ltd on behalf of Royal Pharmaceutical Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Drugs from Natural Sources
Duarte, Filipe Silveira
Duzzioni, Marcelo
Prim, Rafael Luiz
Cardozo, Alcíbia Maia
dos Santos, Claudia Regina
da Silva, Maria Goretti
Shiozawa, Maria Beatriz Cacese
Mendes, Beatriz Garcia
Tizziani, Tiago
Brighente, Inês Maria Costa
Pizzolatti, Moacir Geraldo
de Lima, Thereza Christina Monteiro
Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title_full Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title_fullStr Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title_full_unstemmed Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title_short Psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from Polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
title_sort psychopharmacological effects and safety of styryl‐2‐pyrones and dihydrostyryl‐2‐pyrones‐rich fraction from polygala sabulosa: absence of withdrawal syndrome and tolerance to anxiolytic‐like and anticonvulsant effects
topic Drugs from Natural Sources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099234/
https://www.ncbi.nlm.nih.gov/pubmed/29956326
http://dx.doi.org/10.1111/jphp.12960
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