An optimized FAIRE procedure for low cell numbers in yeast

We report an optimized low‐input FAIRE‐seq (Formaldehyde‐Assisted Isolation of Regulatory Elements‐sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few coloni...

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Detalles Bibliográficos
Autores principales: Segorbe, David, Wilkinson, Derek, Mizeranschi, Alexandru, Hughes, Timothy, Aaløkken, Ragnhild, Váchová, Libuše, Palková, Zdena, Gilfillan, Gregor D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Genome Screen Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099244/
https://www.ncbi.nlm.nih.gov/pubmed/29577419
http://dx.doi.org/10.1002/yea.3316

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099244/
https://www.ncbi.nlm.nih.gov/pubmed/29577419
http://dx.doi.org/10.1002/yea.3316

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