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An optimized FAIRE procedure for low cell numbers in yeast
We report an optimized low‐input FAIRE‐seq (Formaldehyde‐Assisted Isolation of Regulatory Elements‐sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few coloni...
Autores principales: | Segorbe, David, Wilkinson, Derek, Mizeranschi, Alexandru, Hughes, Timothy, Aaløkken, Ragnhild, Váchová, Libuše, Palková, Zdena, Gilfillan, Gregor D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099244/ https://www.ncbi.nlm.nih.gov/pubmed/29577419 http://dx.doi.org/10.1002/yea.3316 |
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