Cargando…

Monolith affinity chromatography for the rapid quantification of a single‐chain variable fragment immunotoxin

We developed a novel analytical method for concentration determination of tandem single‐chain antibody diphtheria toxin (immunotoxin). The method is based on polymethacrylate monoliths with Protein L ligands as the binding moiety. Different buffers were tested for elution of the Protein L‐bound immu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Satzer, Peter, Sommer, Ralf, Paulsson, Johanna, Rodler, Agnes, Zehetner, Romana, Hofstädter, Klaus, Klade, Christoph, Jungbauer, Alois
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Liquid Chromatography
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099420/ https://www.ncbi.nlm.nih.gov/pubmed/29873445 http://dx.doi.org/10.1002/jssc.201800257

Ejemplares similares

High‐capacity protein A affinity chromatography for the fast quantification of antibodies: Two‐wavelength detection expands linear range
por: Satzer, Peter, et al.
Publicado: (2018)

Hydrophobic interaction chromatography of proteins: Studies of unfolding upon adsorption by isothermal titration calorimetry
por: Rodler, Agnes, et al.
Publicado: (2018)

Antibody adsorption in protein‐A affinity chromatography – in situ measurement of nanoscale structure by small‐angle X‐ray scattering
por: Plewka, Jacek, et al.
Publicado: (2018)

Prediction of the performance of pre‐packed purification columns through machine learning
por: Jiang, Qihao, et al.
Publicado: (2022)

Rapid monitoring of high‐mannose glycans during cell culture process of therapeutic monoclonal antibodies using lectin affinity chromatography
por: Kim, Jun, et al.
Publicado: (2022)

Separation of influenza virus‐like particles from baculovirus by polymer‐grafted anion exchanger
por: Reiter, Katrin, et al.
Publicado: (2020)

Melodamide A from Melodorum fruticosum — Quantification using HPLC and one‐step‐isolation by centrifugal partition chromatography
por: Engels, Nora S., et al.
Publicado: (2019)

At‐line multi‐angle light scattering detector for faster process development in enveloped virus‐like particle purification
por: Pereira Aguilar, Patricia, et al.
Publicado: (2019)

Recent applications of retention modelling in liquid chromatography
por: den Uijl, Mimi J., et al.
Publicado: (2020)

Analysis of the California list of pesticides, mycotoxins, and cannabinoids in chocolate using liquid chromatography and low‐pressure gas chromatography‐based platforms
por: Reyes‐Garcés, Nathaly, et al.
Publicado: (2021)

Recent applications of chemometrics in one‐ and two‐dimensional chromatography
por: Bos, Tijmen S., et al.
Publicado: (2020)

Detection challenges in quantitative polymer analysis by liquid chromatography
por: Knol, Wouter C., et al.
Publicado: (2020)

Optimizing separations in online comprehensive two‐dimensional liquid chromatography
por: Pirok, Bob W.J., et al.
Publicado: (2017)

A convenient separation strategy for fungal anthraquinones by centrifugal partition chromatography
por: Hammerle, Fabian, et al.
Publicado: (2022)

Contribution of ionic interactions to stationary phase selectivity in hydrophilic interaction chromatography
por: Gilar, Martin, et al.
Publicado: (2022)

Prediction of peptides retention behavior in reversed‐phase liquid chromatography based on their hydrophobicity
por: Al Musaimi, Othman, et al.
Publicado: (2022)

Determination of Liposomal Cisplatin by High-Performance Liquid Chromatography and Its Application in Pharmacokinetic Studies
por: Toro-Córdova, Alfonso, et al.
Publicado: (2016)

Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products
por: Murisier, Amarande, et al.
Publicado: (2022)

Ultra‐high‐performance supercritical fluid chromatography‐mass spectrometry for the analysis of organic contaminants in sediments
por: Lübeck, Josephine S., et al.
Publicado: (2022)

Characterization of binding by repaglinide and nateglinide with glycated human serum albumin using high‐performance affinity microcolumns
por: Ovbude, Susan T., et al.
Publicado: (2022)

Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest
por: Bouza, Marcos, et al.
Publicado: (2022)

Ultra‐high performance supercritical fluid chromatography of lignin‐derived phenols from alkaline cupric oxide oxidation
por: Sun, Mingzhe, et al.
Publicado: (2016)

Chiral separation using cyclodextrins as mobile phase additives in open‐tubular liquid chromatography with a pseudophase coating
por: Yu, Raymond B., et al.
Publicado: (2022)

Evaluation of hydrophobic‐interaction chromatography resins for purification of antibody‐drug conjugates using a mimetic model with adjustable hydrophobicity
por: Müller, Egbert, et al.
Publicado: (2020)

Profiling and quantitative analysis of underivatized fatty acids in Chlorella vulgaris microalgae by liquid chromatography‐high resolution mass spectrometry
por: Montone, Carmela Maria, et al.
Publicado: (2021)

Characterization of a highly stable zwitterionic hydrophilic interaction chromatography stationary phase based on hybrid organic–inorganic particles
por: Walter, Thomas H., et al.
Publicado: (2022)

Comparison of microcapillary column length and inner diameter investigated with gradient analysis of lipids by ultrahigh‐pressure liquid chromatography‐mass spectrometry
por: Miller, Kelsey E., et al.
Publicado: (2020)

Determination of the polyphenolic content of Ammodaucus leucotrichus Cosson and Durieu by liquid chromatography coupled with mass spectrometry and evaluation of the antioxidant and antiglycation properties
por: Selama, Ginane, et al.
Publicado: (2022)

Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry
por: Oh, Jae‐Hyeon, et al.
Publicado: (2018)

Influence of different hydrophilic interaction liquid chromatography stationary phases on method performance for the determination of highly polar anionic pesticides in complex feed matrices
por: Dias, Jonatan, et al.
Publicado: (2021)

Combination of ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry and network pharmacology to reveal the mechanism of Shengyu Decoction for treating anemia
por: Hu, Yu, et al.
Publicado: (2022)

Quantitative analysis of N‐acylphosphatidylethanolamine molecular species in rat brain using solid‐phase extraction combined with reversed‐phase chromatography and tandem mass spectrometry
por: Triebl, Alexander, et al.
Publicado: (2016)

A novel sensitive analytical method for the simultaneous analysis of vancomycin and teicoplanin in human urine via single high‐performance liquid chromatography coupled with photodiode array and mass spectrometry in series
por: Giakoumaki, Maria, et al.
Publicado: (2022)

Chemical interaction between Paeonia lactiflora and Glycyrrhiza uralensis, the components of Jakyakgamcho-tang, using a validated high-performance liquid chromatography method: Herbal combination and chemical interaction in a decoction
por: Kim, Jung-Hoon, et al.
Publicado: (2014)

A sensitive LC‐MS/MS method for quantification of phenytoin and its major metabolite with application to in vivo investigations of intravenous and intranasal phenytoin delivery
por: Prentice, Richard N., et al.
Publicado: (2022)

LC method for the direct and simultaneous determination of four major furan derivatives in coffee grounds and brews
por: Albouchi, Abdullatif, et al.
Publicado: (2019)

Chromatographic analysis of tryptophan metabolites
por: Sadok, Ilona, et al.
Publicado: (2017)

Elevated viscosities in a simulated moving bed for γ‐aminobutyric acid recovery
por: Schultze‐Jena, A., et al.
Publicado: (2020)

Characterization of a highly stable mixed‐mode reversed‐phase/weak anion‐exchange stationary phase based on hybrid organic/inorganic particles
por: Walter, Thomas H., et al.
Publicado: (2021)

A simple mathematical treatment for predicting linear solvent strength behavior in gradient elution: Application to biomolecules
por: Guillarme, Davy, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_tbn3a80hklltrahntqpm302rpl