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Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview

The neural crest is one of the embryonic structures with the broadest developmental potential in vertebrates. Morphologically, neural crest cells emerge during neurulation in the dorsal folds of the neural tube before undergoing an epithelial‐to‐mesenchymal transition (EMT), delaminating from the ne...

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Autores principales: Debbache, Julien, Parfejevs, Vadims, Sommer, Lukas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099459/
https://www.ncbi.nlm.nih.gov/pubmed/29673028
http://dx.doi.org/10.1002/dvg.23105
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author Debbache, Julien
Parfejevs, Vadims
Sommer, Lukas
author_facet Debbache, Julien
Parfejevs, Vadims
Sommer, Lukas
author_sort Debbache, Julien
collection PubMed
description The neural crest is one of the embryonic structures with the broadest developmental potential in vertebrates. Morphologically, neural crest cells emerge during neurulation in the dorsal folds of the neural tube before undergoing an epithelial‐to‐mesenchymal transition (EMT), delaminating from the neural tube, and migrating to multiple sites in the growing embryo. Neural crest cells generate cell types as diverse as peripheral neurons and glia, melanocytes, and so‐called mesectodermal derivatives that include craniofacial bone and cartilage and smooth muscle cells in cardiovascular structures. In mice, the fate of neural crest cells has been determined mainly by means of transgenesis and genome editing technologies. The most frequently used method relies on the Cre‐loxP system, in which expression of Cre‐recombinase in neural crest cells or their derivatives genetically enables the expression of a Cre‐reporter allele, thus permanently marking neural crest‐derived cells. Here, we provide an overview of the Cre‐driver lines used in the field and discuss to what extent these lines allow precise neural crest stage and lineage‐specific fate mapping.
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spelling pubmed-60994592018-08-24 Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview Debbache, Julien Parfejevs, Vadims Sommer, Lukas Genesis Reviews The neural crest is one of the embryonic structures with the broadest developmental potential in vertebrates. Morphologically, neural crest cells emerge during neurulation in the dorsal folds of the neural tube before undergoing an epithelial‐to‐mesenchymal transition (EMT), delaminating from the neural tube, and migrating to multiple sites in the growing embryo. Neural crest cells generate cell types as diverse as peripheral neurons and glia, melanocytes, and so‐called mesectodermal derivatives that include craniofacial bone and cartilage and smooth muscle cells in cardiovascular structures. In mice, the fate of neural crest cells has been determined mainly by means of transgenesis and genome editing technologies. The most frequently used method relies on the Cre‐loxP system, in which expression of Cre‐recombinase in neural crest cells or their derivatives genetically enables the expression of a Cre‐reporter allele, thus permanently marking neural crest‐derived cells. Here, we provide an overview of the Cre‐driver lines used in the field and discuss to what extent these lines allow precise neural crest stage and lineage‐specific fate mapping. John Wiley and Sons Inc. 2018-04-19 2018 /pmc/articles/PMC6099459/ /pubmed/29673028 http://dx.doi.org/10.1002/dvg.23105 Text en © 2018 The Authors Genesis: The Journal of Genetics and Development Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Reviews
Debbache, Julien
Parfejevs, Vadims
Sommer, Lukas
Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title_full Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title_fullStr Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title_full_unstemmed Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title_short Cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: An overview
title_sort cre‐driver lines used for genetic fate mapping of neural crest cells in the mouse: an overview
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099459/
https://www.ncbi.nlm.nih.gov/pubmed/29673028
http://dx.doi.org/10.1002/dvg.23105
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