Cargando…
The Tryptophan Decarboxylase in Solanum lycopersicum
Melatonin plays an important role in plant growth, development, and environmental stress. In this study, a systematic analysis of tomato tryptophan decarboxylase (SlTrpDC), which is the first enzyme of melatonin biosynthesis, was conducted by integrating structural features, phylogenetic relationshi...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099541/ https://www.ncbi.nlm.nih.gov/pubmed/29695104 http://dx.doi.org/10.3390/molecules23050998 |
_version_ | 1783348688964288512 |
---|---|
author | Pang, Xin Wei, Yanping Cheng, Yuan Pan, Luzhao Ye, Qingjing Wang, Rongqing Ruan, Meiying Zhou, Guozhi Yao, Zhuping Li, Zhimiao Yang, Yuejian Liu, Weicheng Wan, Hongjian |
author_facet | Pang, Xin Wei, Yanping Cheng, Yuan Pan, Luzhao Ye, Qingjing Wang, Rongqing Ruan, Meiying Zhou, Guozhi Yao, Zhuping Li, Zhimiao Yang, Yuejian Liu, Weicheng Wan, Hongjian |
author_sort | Pang, Xin |
collection | PubMed |
description | Melatonin plays an important role in plant growth, development, and environmental stress. In this study, a systematic analysis of tomato tryptophan decarboxylase (SlTrpDC), which is the first enzyme of melatonin biosynthesis, was conducted by integrating structural features, phylogenetic relationships, an exon/intron feature, and a divergent expression profile. The results determined that the tomato genome encoded five members (SlTrpDC1-SlTrpDC5). The phylogenetic relationships indicated that gene expansion was proposed as the major mode of evolution of the TrpDC genes from the different plant algae species to the higher plants species. The analyses of the exon/intron configurations revealed that the intron loss events occurred during the structural evolution of the TrpDCs in plants. Additionally, the RNA-seq and qRT-PCR analysis revealed that the expression of the SlTrpDC3 was high in all of the tested tissues, while the SlTrpDC4 and SlTrpDC5 were not expressed. The expression patterns of the remaining two (SlTrpDC1 and SlTrpDC2) were tissue-specific, which indicated that these genes may play important roles within the different tissues. No expression difference was observed in the tomato plants in response to the biotic stresses. This study will expand the current knowledge of the roles of the TrpDC genes in tomato growth and development. |
format | Online Article Text |
id | pubmed-6099541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60995412018-11-13 The Tryptophan Decarboxylase in Solanum lycopersicum Pang, Xin Wei, Yanping Cheng, Yuan Pan, Luzhao Ye, Qingjing Wang, Rongqing Ruan, Meiying Zhou, Guozhi Yao, Zhuping Li, Zhimiao Yang, Yuejian Liu, Weicheng Wan, Hongjian Molecules Article Melatonin plays an important role in plant growth, development, and environmental stress. In this study, a systematic analysis of tomato tryptophan decarboxylase (SlTrpDC), which is the first enzyme of melatonin biosynthesis, was conducted by integrating structural features, phylogenetic relationships, an exon/intron feature, and a divergent expression profile. The results determined that the tomato genome encoded five members (SlTrpDC1-SlTrpDC5). The phylogenetic relationships indicated that gene expansion was proposed as the major mode of evolution of the TrpDC genes from the different plant algae species to the higher plants species. The analyses of the exon/intron configurations revealed that the intron loss events occurred during the structural evolution of the TrpDCs in plants. Additionally, the RNA-seq and qRT-PCR analysis revealed that the expression of the SlTrpDC3 was high in all of the tested tissues, while the SlTrpDC4 and SlTrpDC5 were not expressed. The expression patterns of the remaining two (SlTrpDC1 and SlTrpDC2) were tissue-specific, which indicated that these genes may play important roles within the different tissues. No expression difference was observed in the tomato plants in response to the biotic stresses. This study will expand the current knowledge of the roles of the TrpDC genes in tomato growth and development. MDPI 2018-04-24 /pmc/articles/PMC6099541/ /pubmed/29695104 http://dx.doi.org/10.3390/molecules23050998 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pang, Xin Wei, Yanping Cheng, Yuan Pan, Luzhao Ye, Qingjing Wang, Rongqing Ruan, Meiying Zhou, Guozhi Yao, Zhuping Li, Zhimiao Yang, Yuejian Liu, Weicheng Wan, Hongjian The Tryptophan Decarboxylase in Solanum lycopersicum |
title | The Tryptophan Decarboxylase in Solanum lycopersicum |
title_full | The Tryptophan Decarboxylase in Solanum lycopersicum |
title_fullStr | The Tryptophan Decarboxylase in Solanum lycopersicum |
title_full_unstemmed | The Tryptophan Decarboxylase in Solanum lycopersicum |
title_short | The Tryptophan Decarboxylase in Solanum lycopersicum |
title_sort | tryptophan decarboxylase in solanum lycopersicum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099541/ https://www.ncbi.nlm.nih.gov/pubmed/29695104 http://dx.doi.org/10.3390/molecules23050998 |
work_keys_str_mv | AT pangxin thetryptophandecarboxylaseinsolanumlycopersicum AT weiyanping thetryptophandecarboxylaseinsolanumlycopersicum AT chengyuan thetryptophandecarboxylaseinsolanumlycopersicum AT panluzhao thetryptophandecarboxylaseinsolanumlycopersicum AT yeqingjing thetryptophandecarboxylaseinsolanumlycopersicum AT wangrongqing thetryptophandecarboxylaseinsolanumlycopersicum AT ruanmeiying thetryptophandecarboxylaseinsolanumlycopersicum AT zhouguozhi thetryptophandecarboxylaseinsolanumlycopersicum AT yaozhuping thetryptophandecarboxylaseinsolanumlycopersicum AT lizhimiao thetryptophandecarboxylaseinsolanumlycopersicum AT yangyuejian thetryptophandecarboxylaseinsolanumlycopersicum AT liuweicheng thetryptophandecarboxylaseinsolanumlycopersicum AT wanhongjian thetryptophandecarboxylaseinsolanumlycopersicum AT pangxin tryptophandecarboxylaseinsolanumlycopersicum AT weiyanping tryptophandecarboxylaseinsolanumlycopersicum AT chengyuan tryptophandecarboxylaseinsolanumlycopersicum AT panluzhao tryptophandecarboxylaseinsolanumlycopersicum AT yeqingjing tryptophandecarboxylaseinsolanumlycopersicum AT wangrongqing tryptophandecarboxylaseinsolanumlycopersicum AT ruanmeiying tryptophandecarboxylaseinsolanumlycopersicum AT zhouguozhi tryptophandecarboxylaseinsolanumlycopersicum AT yaozhuping tryptophandecarboxylaseinsolanumlycopersicum AT lizhimiao tryptophandecarboxylaseinsolanumlycopersicum AT yangyuejian tryptophandecarboxylaseinsolanumlycopersicum AT liuweicheng tryptophandecarboxylaseinsolanumlycopersicum AT wanhongjian tryptophandecarboxylaseinsolanumlycopersicum |