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Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways

Inflammatory bowel disease (IBD) is a notable health problem and may considerably affect the quality of human life. Previously, the protective roles of tryptanthrin (TRYP) against dextran sulfate sodium (DSS) induced colitis has been proved, but the concrete mechanism remained elusive. It has been s...

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Autores principales: Wang, Zheng, Wu, Xue, Wang, Cui-Ling, Wang, Li, Sun, Chen, Zhang, Dong-Bo, Liu, Jian-Li, Liang, Yan-Ni, Tang, Dong-Xin, Tang, Zhi-Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099556/
https://www.ncbi.nlm.nih.gov/pubmed/29724065
http://dx.doi.org/10.3390/molecules23051062
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author Wang, Zheng
Wu, Xue
Wang, Cui-Ling
Wang, Li
Sun, Chen
Zhang, Dong-Bo
Liu, Jian-Li
Liang, Yan-Ni
Tang, Dong-Xin
Tang, Zhi-Shu
author_facet Wang, Zheng
Wu, Xue
Wang, Cui-Ling
Wang, Li
Sun, Chen
Zhang, Dong-Bo
Liu, Jian-Li
Liang, Yan-Ni
Tang, Dong-Xin
Tang, Zhi-Shu
author_sort Wang, Zheng
collection PubMed
description Inflammatory bowel disease (IBD) is a notable health problem and may considerably affect the quality of human life. Previously, the protective roles of tryptanthrin (TRYP) against dextran sulfate sodium (DSS) induced colitis has been proved, but the concrete mechanism remained elusive. It has been suggested that TRYP could diminish the weight loss and improve the health conditions of mice with DSS induced colitis. Hematoxylin and eosin staining revealed that TRYP could improve the histopathological structure of the colon tissue. Two signaling pathways (TNF-α/NF-κBp65 and IL-6/STAT3) were investigated using immunochemistry and western blot. The detected concentrations of the two cytokines TNF-α and IL-6 showed that their levels decreased after TRYP treatment of the colitis. The protein expression level of NF-κBp65 in cytoplasm increased after TRYP treatment of the induced colitis. However, the protein level of NF-κBp65 in the nucleus decreased after administration of TRYP. The expression level of IκBα, the inhibitory protein of NF-κBp65, was tested and the results suggested that TRYP could inhibit the degradation of IκBα. The phosphorylation level of STAT3 was inhibited by TRYP and the expression level of STAT3 and p-STAT3 decreased after administration of TRYP. We conclude that TRYP improves the health condition of mice with DSS induced colitis by regulating the TNF-α/NF-κBp65 and IL-6/STAT3 signaling pathways via inhibiting the degradation of IκBα and the phosphorylation of STAT3.
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spelling pubmed-60995562018-11-13 Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways Wang, Zheng Wu, Xue Wang, Cui-Ling Wang, Li Sun, Chen Zhang, Dong-Bo Liu, Jian-Li Liang, Yan-Ni Tang, Dong-Xin Tang, Zhi-Shu Molecules Article Inflammatory bowel disease (IBD) is a notable health problem and may considerably affect the quality of human life. Previously, the protective roles of tryptanthrin (TRYP) against dextran sulfate sodium (DSS) induced colitis has been proved, but the concrete mechanism remained elusive. It has been suggested that TRYP could diminish the weight loss and improve the health conditions of mice with DSS induced colitis. Hematoxylin and eosin staining revealed that TRYP could improve the histopathological structure of the colon tissue. Two signaling pathways (TNF-α/NF-κBp65 and IL-6/STAT3) were investigated using immunochemistry and western blot. The detected concentrations of the two cytokines TNF-α and IL-6 showed that their levels decreased after TRYP treatment of the colitis. The protein expression level of NF-κBp65 in cytoplasm increased after TRYP treatment of the induced colitis. However, the protein level of NF-κBp65 in the nucleus decreased after administration of TRYP. The expression level of IκBα, the inhibitory protein of NF-κBp65, was tested and the results suggested that TRYP could inhibit the degradation of IκBα. The phosphorylation level of STAT3 was inhibited by TRYP and the expression level of STAT3 and p-STAT3 decreased after administration of TRYP. We conclude that TRYP improves the health condition of mice with DSS induced colitis by regulating the TNF-α/NF-κBp65 and IL-6/STAT3 signaling pathways via inhibiting the degradation of IκBα and the phosphorylation of STAT3. MDPI 2018-05-02 /pmc/articles/PMC6099556/ /pubmed/29724065 http://dx.doi.org/10.3390/molecules23051062 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zheng
Wu, Xue
Wang, Cui-Ling
Wang, Li
Sun, Chen
Zhang, Dong-Bo
Liu, Jian-Li
Liang, Yan-Ni
Tang, Dong-Xin
Tang, Zhi-Shu
Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title_full Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title_fullStr Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title_full_unstemmed Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title_short Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
title_sort tryptanthrin protects mice against dextran sulfate sodium-induced colitis through inhibition of tnf-α/nf-κb and il-6/stat3 pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099556/
https://www.ncbi.nlm.nih.gov/pubmed/29724065
http://dx.doi.org/10.3390/molecules23051062
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