Cargando…

Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity

Panax ginseng is well known for its medicinal functions. As a class of important compound of ginseng, ginsenoside is widely studied around the world. In addition, ginseng glycopeptides also showed good biological activity, but researches in this field are rarely reported. In this study, ginseng glyc...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Haoming, Zhu, Difu, Wang, Ying, Chen, Yinghong, Jiang, Ruizhi, Yu, Peng, Qiu, Zhidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099564/
https://www.ncbi.nlm.nih.gov/pubmed/29857514
http://dx.doi.org/10.3390/molecules23061325
_version_ 1783348694493429760
author Luo, Haoming
Zhu, Difu
Wang, Ying
Chen, Yinghong
Jiang, Ruizhi
Yu, Peng
Qiu, Zhidong
author_facet Luo, Haoming
Zhu, Difu
Wang, Ying
Chen, Yinghong
Jiang, Ruizhi
Yu, Peng
Qiu, Zhidong
author_sort Luo, Haoming
collection PubMed
description Panax ginseng is well known for its medicinal functions. As a class of important compound of ginseng, ginsenoside is widely studied around the world. In addition, ginseng glycopeptides also showed good biological activity, but researches in this field are rarely reported. In this study, ginseng glycopeptides (Gg) were first prepared from Panax ginseng by reflux extracted with 85% ethanol and the following purification with Sephadex G-15 column. Then, the inflammatory pain models induced by carrageenan and the rat pain models induced by Faure Marin were established for research on mechanism of analgesic activities. It is showed that Gg had an obvious inhibiting effect on inflammation and a significant reduction on the Malondialdehyde (MDA) of inflammatory foot tissue. And there were significant differences between moderate to high dose of Gg and model group in Interleukin 1β (IL-1β), Interleukin 2 (IL-2), Interleukin 4 (IL-4), Tumor necrosis factor α (TNF-α) and Histamine. The two models can be preliminarily determined that the analgesic effect of Gg may be peripheral, which mechanism may be related to the dynamic balance between proinflammatory cytokines (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-2, IL-4, and Interleukin 10 (IL-10)). A series of methods were used to study Gg in physical-chemical properties and linking mode of glycoside. The high-resolution mass spectrometry was used for identification of the structure of Gg. Moreover, the structure of 20 major Gg were investigated and identified. The structural analysis of Gg was benefit for the next study on structure-activity relationship.
format Online
Article
Text
id pubmed-6099564
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60995642018-11-13 Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity Luo, Haoming Zhu, Difu Wang, Ying Chen, Yinghong Jiang, Ruizhi Yu, Peng Qiu, Zhidong Molecules Article Panax ginseng is well known for its medicinal functions. As a class of important compound of ginseng, ginsenoside is widely studied around the world. In addition, ginseng glycopeptides also showed good biological activity, but researches in this field are rarely reported. In this study, ginseng glycopeptides (Gg) were first prepared from Panax ginseng by reflux extracted with 85% ethanol and the following purification with Sephadex G-15 column. Then, the inflammatory pain models induced by carrageenan and the rat pain models induced by Faure Marin were established for research on mechanism of analgesic activities. It is showed that Gg had an obvious inhibiting effect on inflammation and a significant reduction on the Malondialdehyde (MDA) of inflammatory foot tissue. And there were significant differences between moderate to high dose of Gg and model group in Interleukin 1β (IL-1β), Interleukin 2 (IL-2), Interleukin 4 (IL-4), Tumor necrosis factor α (TNF-α) and Histamine. The two models can be preliminarily determined that the analgesic effect of Gg may be peripheral, which mechanism may be related to the dynamic balance between proinflammatory cytokines (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-2, IL-4, and Interleukin 10 (IL-10)). A series of methods were used to study Gg in physical-chemical properties and linking mode of glycoside. The high-resolution mass spectrometry was used for identification of the structure of Gg. Moreover, the structure of 20 major Gg were investigated and identified. The structural analysis of Gg was benefit for the next study on structure-activity relationship. MDPI 2018-05-31 /pmc/articles/PMC6099564/ /pubmed/29857514 http://dx.doi.org/10.3390/molecules23061325 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Haoming
Zhu, Difu
Wang, Ying
Chen, Yinghong
Jiang, Ruizhi
Yu, Peng
Qiu, Zhidong
Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title_full Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title_fullStr Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title_full_unstemmed Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title_short Study on the Structure of Ginseng Glycopeptides with Anti-Inflammatory and Analgesic Activity
title_sort study on the structure of ginseng glycopeptides with anti-inflammatory and analgesic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099564/
https://www.ncbi.nlm.nih.gov/pubmed/29857514
http://dx.doi.org/10.3390/molecules23061325
work_keys_str_mv AT luohaoming studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT zhudifu studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT wangying studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT chenyinghong studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT jiangruizhi studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT yupeng studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity
AT qiuzhidong studyonthestructureofginsengglycopeptideswithantiinflammatoryandanalgesicactivity