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Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea

Alkenal double bond reductases (DBRs), capable of catalyzing the NADPH-dependent reduction of the α,β-unsaturated double bond, play key roles in the detoxication of alkenal carbonyls. Here, the isolation and characterization of two DBRs encoded by the liverwort species Marchantia paleacea are descri...

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Autores principales: Wu, Yi-Feng, Zheng, Hong-Bo, Liu, Xin-Yan, Cheng, Ai-Xia, Lou, Hong-Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099575/
https://www.ncbi.nlm.nih.gov/pubmed/29973530
http://dx.doi.org/10.3390/molecules23071630
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author Wu, Yi-Feng
Zheng, Hong-Bo
Liu, Xin-Yan
Cheng, Ai-Xia
Lou, Hong-Xiang
author_facet Wu, Yi-Feng
Zheng, Hong-Bo
Liu, Xin-Yan
Cheng, Ai-Xia
Lou, Hong-Xiang
author_sort Wu, Yi-Feng
collection PubMed
description Alkenal double bond reductases (DBRs), capable of catalyzing the NADPH-dependent reduction of the α,β-unsaturated double bond, play key roles in the detoxication of alkenal carbonyls. Here, the isolation and characterization of two DBRs encoded by the liverwort species Marchantia paleacea are described. The two DBRs share a relatively low similarity, and phylogenetic analysis indicated that MpMDBRL is more closely related to microbial DBRs than to other plant DBRs, while MpDBR shares common ancestry with typical plant DBRs. Both DBR proteins exhibited hydrogenation ability towards hydroxycinnamyl aldehydes; however, their temperature optimums were strikingly different. MpMDBRL demonstrated slightly weaker catalytic efficiency compared to MpDBR, and the structural models of their active binding sites to the substrate may provide a parsimonious explanation. Furthermore, both DBRs significantly responded to phytohormone treatment. In conclusion, M. paleacea produces two distinct types of functional DBRs, both of which participate in the protection against environmental stress in liverwort. The presence of a microbial type of DBR in a plant is herein reported for the first time.
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spelling pubmed-60995752018-11-13 Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea Wu, Yi-Feng Zheng, Hong-Bo Liu, Xin-Yan Cheng, Ai-Xia Lou, Hong-Xiang Molecules Article Alkenal double bond reductases (DBRs), capable of catalyzing the NADPH-dependent reduction of the α,β-unsaturated double bond, play key roles in the detoxication of alkenal carbonyls. Here, the isolation and characterization of two DBRs encoded by the liverwort species Marchantia paleacea are described. The two DBRs share a relatively low similarity, and phylogenetic analysis indicated that MpMDBRL is more closely related to microbial DBRs than to other plant DBRs, while MpDBR shares common ancestry with typical plant DBRs. Both DBR proteins exhibited hydrogenation ability towards hydroxycinnamyl aldehydes; however, their temperature optimums were strikingly different. MpMDBRL demonstrated slightly weaker catalytic efficiency compared to MpDBR, and the structural models of their active binding sites to the substrate may provide a parsimonious explanation. Furthermore, both DBRs significantly responded to phytohormone treatment. In conclusion, M. paleacea produces two distinct types of functional DBRs, both of which participate in the protection against environmental stress in liverwort. The presence of a microbial type of DBR in a plant is herein reported for the first time. MDPI 2018-07-04 /pmc/articles/PMC6099575/ /pubmed/29973530 http://dx.doi.org/10.3390/molecules23071630 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Yi-Feng
Zheng, Hong-Bo
Liu, Xin-Yan
Cheng, Ai-Xia
Lou, Hong-Xiang
Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title_full Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title_fullStr Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title_full_unstemmed Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title_short Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
title_sort molecular diversity of alkenal double bond reductases in the liverwort marchantia paleacea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099575/
https://www.ncbi.nlm.nih.gov/pubmed/29973530
http://dx.doi.org/10.3390/molecules23071630
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