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Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species

Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different...

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Autores principales: Zhang, Huamei, Wang, Hui, Wei, Juan, Chen, Xiaopeng, Sun, Mengjie, Ouyang, Huizi, Hao, Jia, Chang, Yanxu, Dou, Zhiying, He, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099698/
https://www.ncbi.nlm.nih.gov/pubmed/29986486
http://dx.doi.org/10.3390/molecules23071656
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author Zhang, Huamei
Wang, Hui
Wei, Juan
Chen, Xiaopeng
Sun, Mengjie
Ouyang, Huizi
Hao, Jia
Chang, Yanxu
Dou, Zhiying
He, Jun
author_facet Zhang, Huamei
Wang, Hui
Wei, Juan
Chen, Xiaopeng
Sun, Mengjie
Ouyang, Huizi
Hao, Jia
Chang, Yanxu
Dou, Zhiying
He, Jun
author_sort Zhang, Huamei
collection PubMed
description Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents.
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spelling pubmed-60996982018-11-13 Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species Zhang, Huamei Wang, Hui Wei, Juan Chen, Xiaopeng Sun, Mengjie Ouyang, Huizi Hao, Jia Chang, Yanxu Dou, Zhiying He, Jun Molecules Article Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents. MDPI 2018-07-07 /pmc/articles/PMC6099698/ /pubmed/29986486 http://dx.doi.org/10.3390/molecules23071656 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Huamei
Wang, Hui
Wei, Juan
Chen, Xiaopeng
Sun, Mengjie
Ouyang, Huizi
Hao, Jia
Chang, Yanxu
Dou, Zhiying
He, Jun
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title_full Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title_fullStr Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title_full_unstemmed Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title_short Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
title_sort comparison of the active compositions between raw and processed epimedium from different species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099698/
https://www.ncbi.nlm.nih.gov/pubmed/29986486
http://dx.doi.org/10.3390/molecules23071656
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