Cargando…
Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099941/ https://www.ncbi.nlm.nih.gov/pubmed/29895732 http://dx.doi.org/10.3390/molecules23061418 |
_version_ | 1783348764766896128 |
---|---|
author | Stivers, Nicole S. Islam, Ashraful Reyes-Reyes, Elsa M. Casson, Lavona K. Aponte, José C. Vaisberg, Abraham J. Hammond, Gerald B. Bates, Paula J. |
author_facet | Stivers, Nicole S. Islam, Ashraful Reyes-Reyes, Elsa M. Casson, Lavona K. Aponte, José C. Vaisberg, Abraham J. Hammond, Gerald B. Bates, Paula J. |
author_sort | Stivers, Nicole S. |
collection | PubMed |
description | We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G(2)/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells. |
format | Online Article Text |
id | pubmed-6099941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60999412018-11-13 Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death Stivers, Nicole S. Islam, Ashraful Reyes-Reyes, Elsa M. Casson, Lavona K. Aponte, José C. Vaisberg, Abraham J. Hammond, Gerald B. Bates, Paula J. Molecules Article We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G(2)/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells. MDPI 2018-06-12 /pmc/articles/PMC6099941/ /pubmed/29895732 http://dx.doi.org/10.3390/molecules23061418 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stivers, Nicole S. Islam, Ashraful Reyes-Reyes, Elsa M. Casson, Lavona K. Aponte, José C. Vaisberg, Abraham J. Hammond, Gerald B. Bates, Paula J. Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title | Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title_full | Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title_fullStr | Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title_full_unstemmed | Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title_short | Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death |
title_sort | plagiochiline a inhibits cytokinetic abscission and induces cell death |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099941/ https://www.ncbi.nlm.nih.gov/pubmed/29895732 http://dx.doi.org/10.3390/molecules23061418 |
work_keys_str_mv | AT stiversnicoles plagiochilineainhibitscytokineticabscissionandinducescelldeath AT islamashraful plagiochilineainhibitscytokineticabscissionandinducescelldeath AT reyesreyeselsam plagiochilineainhibitscytokineticabscissionandinducescelldeath AT cassonlavonak plagiochilineainhibitscytokineticabscissionandinducescelldeath AT apontejosec plagiochilineainhibitscytokineticabscissionandinducescelldeath AT vaisbergabrahamj plagiochilineainhibitscytokineticabscissionandinducescelldeath AT hammondgeraldb plagiochilineainhibitscytokineticabscissionandinducescelldeath AT batespaulaj plagiochilineainhibitscytokineticabscissionandinducescelldeath |