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Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death

We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as...

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Autores principales: Stivers, Nicole S., Islam, Ashraful, Reyes-Reyes, Elsa M., Casson, Lavona K., Aponte, José C., Vaisberg, Abraham J., Hammond, Gerald B., Bates, Paula J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099941/
https://www.ncbi.nlm.nih.gov/pubmed/29895732
http://dx.doi.org/10.3390/molecules23061418
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author Stivers, Nicole S.
Islam, Ashraful
Reyes-Reyes, Elsa M.
Casson, Lavona K.
Aponte, José C.
Vaisberg, Abraham J.
Hammond, Gerald B.
Bates, Paula J.
author_facet Stivers, Nicole S.
Islam, Ashraful
Reyes-Reyes, Elsa M.
Casson, Lavona K.
Aponte, José C.
Vaisberg, Abraham J.
Hammond, Gerald B.
Bates, Paula J.
author_sort Stivers, Nicole S.
collection PubMed
description We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G(2)/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells.
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spelling pubmed-60999412018-11-13 Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death Stivers, Nicole S. Islam, Ashraful Reyes-Reyes, Elsa M. Casson, Lavona K. Aponte, José C. Vaisberg, Abraham J. Hammond, Gerald B. Bates, Paula J. Molecules Article We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G(2)/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells. MDPI 2018-06-12 /pmc/articles/PMC6099941/ /pubmed/29895732 http://dx.doi.org/10.3390/molecules23061418 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stivers, Nicole S.
Islam, Ashraful
Reyes-Reyes, Elsa M.
Casson, Lavona K.
Aponte, José C.
Vaisberg, Abraham J.
Hammond, Gerald B.
Bates, Paula J.
Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title_full Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title_fullStr Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title_full_unstemmed Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title_short Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death
title_sort plagiochiline a inhibits cytokinetic abscission and induces cell death
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099941/
https://www.ncbi.nlm.nih.gov/pubmed/29895732
http://dx.doi.org/10.3390/molecules23061418
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