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MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation

Background: In the search for novel antidepressive drug candidates, bioguided fractionation of nonpolar constituents present in the oleoresin from ginger rhizomes (Zingiber officinale Roscoe, Zingiberaceae) was performed. This particular direction of the research was chosen due to the existing repor...

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Autores principales: Kukula-Koch, Wirginia, Koch, Wojciech, Czernicka, Lidia, Głowniak, Kazimierz, Asakawa, Yoshinori, Umeyama, Akemi, Marzec, Zbigniew, Kuzuhara, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099963/
https://www.ncbi.nlm.nih.gov/pubmed/29844252
http://dx.doi.org/10.3390/molecules23061301
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author Kukula-Koch, Wirginia
Koch, Wojciech
Czernicka, Lidia
Głowniak, Kazimierz
Asakawa, Yoshinori
Umeyama, Akemi
Marzec, Zbigniew
Kuzuhara, Takashi
author_facet Kukula-Koch, Wirginia
Koch, Wojciech
Czernicka, Lidia
Głowniak, Kazimierz
Asakawa, Yoshinori
Umeyama, Akemi
Marzec, Zbigniew
Kuzuhara, Takashi
author_sort Kukula-Koch, Wirginia
collection PubMed
description Background: In the search for novel antidepressive drug candidates, bioguided fractionation of nonpolar constituents present in the oleoresin from ginger rhizomes (Zingiber officinale Roscoe, Zingiberaceae) was performed. This particular direction of the research was chosen due to the existing reports on the antidepressive properties of ginger total extract. The search for individual metabolites acting as MAO-A inhibitors, which correspond to the apparent effect of the total extract, is the subject of this work. Methods: Hexane extracts from ginger rhizomes were fractionated by using column chromatography (including silica gel impregnated with silver nitrate) and semi-preparative high-performance chromatography. For the activity assessment, an in vitro monoamine oxidase A (MAO-A) inhibition luminescence assay was performed on 10 purified terpenes: 1,8-cineole, α-citronellal, geraniol, β-sesquiphellandrene, γ-terpinen, geranyl acetate, isobornyl acetate, terpinen-4-ol, (E,E)-α-farnesene, and α-zingiberene. Results: Geraniol and (−)-terpinen-4-ol were found to be the strongest enzyme inhibitors with inhibition of 44.1% and 42.5%, respectively, at a concentration of 125 µg/mL. No differences in the inhibition potential were observed for the different groups of terpenes: sesquiterpenes, monoterpenes, or sesquiterpene alcohols; however, the two most active compounds contained a hydroxyl moiety. Conclusions: Terpene constituents from ginger’s extract were found to exhibit moderate inhibitory properties against the MAO-A enzyme in in vitro tests.
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spelling pubmed-60999632018-11-13 MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation Kukula-Koch, Wirginia Koch, Wojciech Czernicka, Lidia Głowniak, Kazimierz Asakawa, Yoshinori Umeyama, Akemi Marzec, Zbigniew Kuzuhara, Takashi Molecules Communication Background: In the search for novel antidepressive drug candidates, bioguided fractionation of nonpolar constituents present in the oleoresin from ginger rhizomes (Zingiber officinale Roscoe, Zingiberaceae) was performed. This particular direction of the research was chosen due to the existing reports on the antidepressive properties of ginger total extract. The search for individual metabolites acting as MAO-A inhibitors, which correspond to the apparent effect of the total extract, is the subject of this work. Methods: Hexane extracts from ginger rhizomes were fractionated by using column chromatography (including silica gel impregnated with silver nitrate) and semi-preparative high-performance chromatography. For the activity assessment, an in vitro monoamine oxidase A (MAO-A) inhibition luminescence assay was performed on 10 purified terpenes: 1,8-cineole, α-citronellal, geraniol, β-sesquiphellandrene, γ-terpinen, geranyl acetate, isobornyl acetate, terpinen-4-ol, (E,E)-α-farnesene, and α-zingiberene. Results: Geraniol and (−)-terpinen-4-ol were found to be the strongest enzyme inhibitors with inhibition of 44.1% and 42.5%, respectively, at a concentration of 125 µg/mL. No differences in the inhibition potential were observed for the different groups of terpenes: sesquiterpenes, monoterpenes, or sesquiterpene alcohols; however, the two most active compounds contained a hydroxyl moiety. Conclusions: Terpene constituents from ginger’s extract were found to exhibit moderate inhibitory properties against the MAO-A enzyme in in vitro tests. MDPI 2018-05-29 /pmc/articles/PMC6099963/ /pubmed/29844252 http://dx.doi.org/10.3390/molecules23061301 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kukula-Koch, Wirginia
Koch, Wojciech
Czernicka, Lidia
Głowniak, Kazimierz
Asakawa, Yoshinori
Umeyama, Akemi
Marzec, Zbigniew
Kuzuhara, Takashi
MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title_full MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title_fullStr MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title_full_unstemmed MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title_short MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation
title_sort mao-a inhibitory potential of terpene constituents from ginger rhizomes—a bioactivity guided fractionation
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099963/
https://www.ncbi.nlm.nih.gov/pubmed/29844252
http://dx.doi.org/10.3390/molecules23061301
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