Validated (1)H and (13)C Nuclear Magnetic Resonance Methods for the Quantitative Determination of Glycerol in Drug Injections

In the current study, we employed high-resolution proton and carbon nuclear magnetic resonance spectroscopy ((1)H and (13)C NMR) for quantitative analysis of glycerol in drug injections without any complex pre-treatment or derivatization on samples. The established methods were validated with good specificity, linearity, accuracy, precision, stability, and repeatability. Our results revealed that the contents of glycerol were convenient to calculate directly via the integration ratios of peak areas with an internal standard in (1)H NMR spectra, while the integration of peak heights were proper for (13)C NMR in combination with an external calibration of glycerol. The developed methods were both successfully applied in drug injections. Quantitative NMR methods showed an extensive prospect for glycerol determination in various liquid samples.