Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresi...

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Detalles Bibliográficos
Autores principales: Chen, Zhe, Ding, Zhaoyang, Zhang, Guangya, Tian, Leilei, Zhang, Xuanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100176/
https://www.ncbi.nlm.nih.gov/pubmed/30011929
http://dx.doi.org/10.3390/molecules23071725
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author Chen, Zhe
Ding, Zhaoyang
Zhang, Guangya
Tian, Leilei
Zhang, Xuanjun
author_facet Chen, Zhe
Ding, Zhaoyang
Zhang, Guangya
Tian, Leilei
Zhang, Xuanjun
author_sort Chen, Zhe
collection PubMed
description In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresis, UV-vis absorption, and fluorescence emission spectroscopy. ELPs40-TPE possessed both amphiphilicity and the features of an AIE, and the fluorescence intensity was dependent on the local temperature. The Hela cells imaging indicated that ELPs40-TPE has great potential for bio-imaging applications because of its advantages of high fluorescence intensity, good water-solubility, and remarkable biocompatibility.
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spelling pubmed-61001762018-11-13 Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing Chen, Zhe Ding, Zhaoyang Zhang, Guangya Tian, Leilei Zhang, Xuanjun Molecules Article In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresis, UV-vis absorption, and fluorescence emission spectroscopy. ELPs40-TPE possessed both amphiphilicity and the features of an AIE, and the fluorescence intensity was dependent on the local temperature. The Hela cells imaging indicated that ELPs40-TPE has great potential for bio-imaging applications because of its advantages of high fluorescence intensity, good water-solubility, and remarkable biocompatibility. MDPI 2018-07-14 /pmc/articles/PMC6100176/ /pubmed/30011929 http://dx.doi.org/10.3390/molecules23071725 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Zhe
Ding, Zhaoyang
Zhang, Guangya
Tian, Leilei
Zhang, Xuanjun
Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title_full Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title_fullStr Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title_full_unstemmed Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title_short Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing
title_sort construction of thermo-responsive elastin-like polypeptides (elps)-aggregation-induced-emission (aie) conjugates for temperature sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100176/
https://www.ncbi.nlm.nih.gov/pubmed/30011929
http://dx.doi.org/10.3390/molecules23071725
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