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Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies

Bacterial infections represent a serious threat in modern medicine. In particular, biofilm treatment in clinical settings is challenging, as biofilms are very resistant to conventional antibiotic therapy and may spread infecting other tissues. To address this problem, biosensing technologies are eme...

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Autores principales: Hoyos-Nogués, Mireia, Gil, F. J., Mas-Moruno, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100210/
https://www.ncbi.nlm.nih.gov/pubmed/29996565
http://dx.doi.org/10.3390/molecules23071683
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author Hoyos-Nogués, Mireia
Gil, F. J.
Mas-Moruno, Carlos
author_facet Hoyos-Nogués, Mireia
Gil, F. J.
Mas-Moruno, Carlos
author_sort Hoyos-Nogués, Mireia
collection PubMed
description Bacterial infections represent a serious threat in modern medicine. In particular, biofilm treatment in clinical settings is challenging, as biofilms are very resistant to conventional antibiotic therapy and may spread infecting other tissues. To address this problem, biosensing technologies are emerging as a powerful solution to detect and identify bacterial pathogens at the very early stages of the infection, thus allowing rapid and effective treatments before biofilms are formed. Biosensors typically consist of two main parts, a biorecognition moiety that interacts with the target (i.e., bacteria) and a platform that transduces such interaction into a measurable signal. This review will focus on the development of impedimetric biosensors using antimicrobial peptides (AMPs) as biorecognition elements. AMPs belong to the innate immune system of living organisms and are very effective in interacting with bacterial membranes. They offer unique advantages compared to other classical bioreceptor molecules such as enzymes or antibodies. Moreover, impedance-based sensors allow the development of label-free, rapid, sensitive, specific and cost-effective sensing platforms. In summary, AMPs and impedimetric transducers combine excellent properties to produce robust biosensors for the early detection of bacterial infections.
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spelling pubmed-61002102018-11-13 Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies Hoyos-Nogués, Mireia Gil, F. J. Mas-Moruno, Carlos Molecules Review Bacterial infections represent a serious threat in modern medicine. In particular, biofilm treatment in clinical settings is challenging, as biofilms are very resistant to conventional antibiotic therapy and may spread infecting other tissues. To address this problem, biosensing technologies are emerging as a powerful solution to detect and identify bacterial pathogens at the very early stages of the infection, thus allowing rapid and effective treatments before biofilms are formed. Biosensors typically consist of two main parts, a biorecognition moiety that interacts with the target (i.e., bacteria) and a platform that transduces such interaction into a measurable signal. This review will focus on the development of impedimetric biosensors using antimicrobial peptides (AMPs) as biorecognition elements. AMPs belong to the innate immune system of living organisms and are very effective in interacting with bacterial membranes. They offer unique advantages compared to other classical bioreceptor molecules such as enzymes or antibodies. Moreover, impedance-based sensors allow the development of label-free, rapid, sensitive, specific and cost-effective sensing platforms. In summary, AMPs and impedimetric transducers combine excellent properties to produce robust biosensors for the early detection of bacterial infections. MDPI 2018-07-10 /pmc/articles/PMC6100210/ /pubmed/29996565 http://dx.doi.org/10.3390/molecules23071683 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Hoyos-Nogués, Mireia
Gil, F. J.
Mas-Moruno, Carlos
Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title_full Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title_fullStr Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title_full_unstemmed Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title_short Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies
title_sort antimicrobial peptides: powerful biorecognition elements to detect bacteria in biosensing technologies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100210/
https://www.ncbi.nlm.nih.gov/pubmed/29996565
http://dx.doi.org/10.3390/molecules23071683
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