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Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method

Qishen granules (QSG) have beneficial therapeutic effects for heart failure, but the effects of decomposed recipes, including Wenyang Yiqi Huoxue (WYH) and Qingre Jiedu (QJ), are not clear. In this study, the efficacy of WYH and QJ on heart failure is evaluated by using transverse aortic constrictio...

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Autores principales: Ren, Weiquan, Gao, Sheng, Zhang, Huimin, Ren, Yinglu, Yu, Xue, Lin, Weili, Guo, Shuzhen, Zhu, Ruixin, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100320/
https://www.ncbi.nlm.nih.gov/pubmed/30041436
http://dx.doi.org/10.3390/molecules23071829
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author Ren, Weiquan
Gao, Sheng
Zhang, Huimin
Ren, Yinglu
Yu, Xue
Lin, Weili
Guo, Shuzhen
Zhu, Ruixin
Wang, Wei
author_facet Ren, Weiquan
Gao, Sheng
Zhang, Huimin
Ren, Yinglu
Yu, Xue
Lin, Weili
Guo, Shuzhen
Zhu, Ruixin
Wang, Wei
author_sort Ren, Weiquan
collection PubMed
description Qishen granules (QSG) have beneficial therapeutic effects for heart failure, but the effects of decomposed recipes, including Wenyang Yiqi Huoxue (WYH) and Qingre Jiedu (QJ), are not clear. In this study, the efficacy of WYH and QJ on heart failure is evaluated by using transverse aortic constriction (TAC) induced mice and the significantly changed genes in heart tissues were screened with a DNA array. Furthermore, a new quantitative pathway analysis tool is developed to evaluate the differences of pathways in different groups and to identify the pharmacological contributions of the decomposed recipes. Finally, the related genes in the significantly changed pathways are verified by a real-time polymerase chain reaction and a Western blot. Our data show that both QJ and WYH improve the left ventricular ejection fraction, which explain their contributions to protect against heart failure. In the energy metabolism, QJ achieves the therapeutic effects of QSG through nicotinamide nucleotide transhydrogenase (Nnt)-mediated mechanisms. In ventricular remodeling and inflammation reactions, QJ and WYH undertake the therapeutic effects through 5′-nucleotidase ecto (Nt5e)-mediated mechanisms. Together, QJ and WYH constitute the therapeutic effects of QSG and play important roles in myocardial energy metabolism and inflammation, which can exert therapeutic effects for heart failure.
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spelling pubmed-61003202018-11-13 Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method Ren, Weiquan Gao, Sheng Zhang, Huimin Ren, Yinglu Yu, Xue Lin, Weili Guo, Shuzhen Zhu, Ruixin Wang, Wei Molecules Article Qishen granules (QSG) have beneficial therapeutic effects for heart failure, but the effects of decomposed recipes, including Wenyang Yiqi Huoxue (WYH) and Qingre Jiedu (QJ), are not clear. In this study, the efficacy of WYH and QJ on heart failure is evaluated by using transverse aortic constriction (TAC) induced mice and the significantly changed genes in heart tissues were screened with a DNA array. Furthermore, a new quantitative pathway analysis tool is developed to evaluate the differences of pathways in different groups and to identify the pharmacological contributions of the decomposed recipes. Finally, the related genes in the significantly changed pathways are verified by a real-time polymerase chain reaction and a Western blot. Our data show that both QJ and WYH improve the left ventricular ejection fraction, which explain their contributions to protect against heart failure. In the energy metabolism, QJ achieves the therapeutic effects of QSG through nicotinamide nucleotide transhydrogenase (Nnt)-mediated mechanisms. In ventricular remodeling and inflammation reactions, QJ and WYH undertake the therapeutic effects through 5′-nucleotidase ecto (Nt5e)-mediated mechanisms. Together, QJ and WYH constitute the therapeutic effects of QSG and play important roles in myocardial energy metabolism and inflammation, which can exert therapeutic effects for heart failure. MDPI 2018-07-23 /pmc/articles/PMC6100320/ /pubmed/30041436 http://dx.doi.org/10.3390/molecules23071829 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ren, Weiquan
Gao, Sheng
Zhang, Huimin
Ren, Yinglu
Yu, Xue
Lin, Weili
Guo, Shuzhen
Zhu, Ruixin
Wang, Wei
Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title_full Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title_fullStr Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title_full_unstemmed Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title_short Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method
title_sort decomposing the mechanism of qishen granules in the treatment of heart failure by a quantitative pathway analysis method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100320/
https://www.ncbi.nlm.nih.gov/pubmed/30041436
http://dx.doi.org/10.3390/molecules23071829
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