Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27

Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity an...

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Detalles Bibliográficos
Autores principales: Sun, Jing, Wang, Shizeng, Li, Wenna, Li, Ruimin, Chen, Sheng, Ri, Hyon Il, Kim, Tae Mun, Kang, Myong Su, Sun, Lu, Sun, Xinxiao, Yuan, Qipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100327/
https://www.ncbi.nlm.nih.gov/pubmed/29734676
http://dx.doi.org/10.3390/molecules23051087
Descripción
Sumario:Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity and the stability of TtTreS against pH and temperature were significantly improved by immobilization. Enzyme immobilization also led to a lower concentration of byproduct glucose, which reduces byproduct inhibition of TtTreS. The immobilized TtTreS still retained 81% of its initial trehalose yield after 22 cycles of enzymatic reactions. The immobilized TtTreS exhibited high operational stability and remarkable reusability, indicating that it is promising for industrial applications.

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