Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27

Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity an...

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Autores principales: Sun, Jing, Wang, Shizeng, Li, Wenna, Li, Ruimin, Chen, Sheng, Ri, Hyon Il, Kim, Tae Mun, Kang, Myong Su, Sun, Lu, Sun, Xinxiao, Yuan, Qipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100327/
https://www.ncbi.nlm.nih.gov/pubmed/29734676
http://dx.doi.org/10.3390/molecules23051087
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author Sun, Jing
Wang, Shizeng
Li, Wenna
Li, Ruimin
Chen, Sheng
Ri, Hyon Il
Kim, Tae Mun
Kang, Myong Su
Sun, Lu
Sun, Xinxiao
Yuan, Qipeng
author_facet Sun, Jing
Wang, Shizeng
Li, Wenna
Li, Ruimin
Chen, Sheng
Ri, Hyon Il
Kim, Tae Mun
Kang, Myong Su
Sun, Lu
Sun, Xinxiao
Yuan, Qipeng
author_sort Sun, Jing
collection PubMed
description Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity and the stability of TtTreS against pH and temperature were significantly improved by immobilization. Enzyme immobilization also led to a lower concentration of byproduct glucose, which reduces byproduct inhibition of TtTreS. The immobilized TtTreS still retained 81% of its initial trehalose yield after 22 cycles of enzymatic reactions. The immobilized TtTreS exhibited high operational stability and remarkable reusability, indicating that it is promising for industrial applications.
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spelling pubmed-61003272018-11-13 Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27 Sun, Jing Wang, Shizeng Li, Wenna Li, Ruimin Chen, Sheng Ri, Hyon Il Kim, Tae Mun Kang, Myong Su Sun, Lu Sun, Xinxiao Yuan, Qipeng Molecules Communication Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity and the stability of TtTreS against pH and temperature were significantly improved by immobilization. Enzyme immobilization also led to a lower concentration of byproduct glucose, which reduces byproduct inhibition of TtTreS. The immobilized TtTreS still retained 81% of its initial trehalose yield after 22 cycles of enzymatic reactions. The immobilized TtTreS exhibited high operational stability and remarkable reusability, indicating that it is promising for industrial applications. MDPI 2018-05-04 /pmc/articles/PMC6100327/ /pubmed/29734676 http://dx.doi.org/10.3390/molecules23051087 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Sun, Jing
Wang, Shizeng
Li, Wenna
Li, Ruimin
Chen, Sheng
Ri, Hyon Il
Kim, Tae Mun
Kang, Myong Su
Sun, Lu
Sun, Xinxiao
Yuan, Qipeng
Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title_full Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title_fullStr Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title_full_unstemmed Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title_short Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27
title_sort improvement of trehalose production by immobilized trehalose synthase from thermus thermophilus hb27
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100327/
https://www.ncbi.nlm.nih.gov/pubmed/29734676
http://dx.doi.org/10.3390/molecules23051087
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