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Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus

Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid con...

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Autores principales: Wang, Yue, Meng, Guisheng, Chen, Sailing, Chen, Yajie, Jiang, Jinjin, Wang, You-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100393/
https://www.ncbi.nlm.nih.gov/pubmed/30037115
http://dx.doi.org/10.3390/molecules23071815
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author Wang, Yue
Meng, Guisheng
Chen, Sailing
Chen, Yajie
Jiang, Jinjin
Wang, You-Ping
author_facet Wang, Yue
Meng, Guisheng
Chen, Sailing
Chen, Yajie
Jiang, Jinjin
Wang, You-Ping
author_sort Wang, Yue
collection PubMed
description Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 ± 0.97 mg EE/g phenolics and 3.78 ± 0.05 mg EE/g flavonoids) than yellow seeds (2.80 ± 0.13 mg/g phenolics and 0.83 ± 0.01 mg/g flavonoids). HPLC–DAD–ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded B. napus, including kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin-3-O-sophoroside, procyanidin B2 ([DP 2]), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 ± 69.98 μmol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 ± 2.40 μmol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded B. napus compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded B. napus.
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spelling pubmed-61003932018-11-13 Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus Wang, Yue Meng, Guisheng Chen, Sailing Chen, Yajie Jiang, Jinjin Wang, You-Ping Molecules Article Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 ± 0.97 mg EE/g phenolics and 3.78 ± 0.05 mg EE/g flavonoids) than yellow seeds (2.80 ± 0.13 mg/g phenolics and 0.83 ± 0.01 mg/g flavonoids). HPLC–DAD–ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded B. napus, including kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin-3-O-sophoroside, procyanidin B2 ([DP 2]), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 ± 69.98 μmol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 ± 2.40 μmol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded B. napus compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded B. napus. MDPI 2018-07-21 /pmc/articles/PMC6100393/ /pubmed/30037115 http://dx.doi.org/10.3390/molecules23071815 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yue
Meng, Guisheng
Chen, Sailing
Chen, Yajie
Jiang, Jinjin
Wang, You-Ping
Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title_full Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title_fullStr Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title_full_unstemmed Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title_short Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
title_sort correlation analysis of phenolic contents and antioxidation in yellow- and black-seeded brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100393/
https://www.ncbi.nlm.nih.gov/pubmed/30037115
http://dx.doi.org/10.3390/molecules23071815
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