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Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus
Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid con...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100393/ https://www.ncbi.nlm.nih.gov/pubmed/30037115 http://dx.doi.org/10.3390/molecules23071815 |
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author | Wang, Yue Meng, Guisheng Chen, Sailing Chen, Yajie Jiang, Jinjin Wang, You-Ping |
author_facet | Wang, Yue Meng, Guisheng Chen, Sailing Chen, Yajie Jiang, Jinjin Wang, You-Ping |
author_sort | Wang, Yue |
collection | PubMed |
description | Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 ± 0.97 mg EE/g phenolics and 3.78 ± 0.05 mg EE/g flavonoids) than yellow seeds (2.80 ± 0.13 mg/g phenolics and 0.83 ± 0.01 mg/g flavonoids). HPLC–DAD–ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded B. napus, including kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin-3-O-sophoroside, procyanidin B2 ([DP 2]), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 ± 69.98 μmol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 ± 2.40 μmol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded B. napus compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded B. napus. |
format | Online Article Text |
id | pubmed-6100393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61003932018-11-13 Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus Wang, Yue Meng, Guisheng Chen, Sailing Chen, Yajie Jiang, Jinjin Wang, You-Ping Molecules Article Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 ± 0.97 mg EE/g phenolics and 3.78 ± 0.05 mg EE/g flavonoids) than yellow seeds (2.80 ± 0.13 mg/g phenolics and 0.83 ± 0.01 mg/g flavonoids). HPLC–DAD–ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded B. napus, including kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin-3-O-sophoroside, procyanidin B2 ([DP 2]), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 ± 69.98 μmol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 ± 2.40 μmol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded B. napus compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded B. napus. MDPI 2018-07-21 /pmc/articles/PMC6100393/ /pubmed/30037115 http://dx.doi.org/10.3390/molecules23071815 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yue Meng, Guisheng Chen, Sailing Chen, Yajie Jiang, Jinjin Wang, You-Ping Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title | Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title_full | Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title_fullStr | Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title_full_unstemmed | Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title_short | Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-Seeded Brassica napus |
title_sort | correlation analysis of phenolic contents and antioxidation in yellow- and black-seeded brassica napus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100393/ https://www.ncbi.nlm.nih.gov/pubmed/30037115 http://dx.doi.org/10.3390/molecules23071815 |
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