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Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans
Osmanthus fragrans, or “RiXiangGui”, is an ornamental, woody, evergreen plant that is cultivated widely because it blooms recurrently and emits a strong fragrance. Recently, the germplasm resources, classification, and aroma compositions of O. fragrans have been investigated. However, the molecular...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100529/ https://www.ncbi.nlm.nih.gov/pubmed/30004428 http://dx.doi.org/10.3390/molecules23071604 |
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author | Yang, Xiu-Lian Li, Hai-Yan Yue, Yuan-Zheng Ding, Wen-Jie Xu, Chen Shi, Ting-Ting Chen, Gong-Wei Wang, Liang-Gui |
author_facet | Yang, Xiu-Lian Li, Hai-Yan Yue, Yuan-Zheng Ding, Wen-Jie Xu, Chen Shi, Ting-Ting Chen, Gong-Wei Wang, Liang-Gui |
author_sort | Yang, Xiu-Lian |
collection | PubMed |
description | Osmanthus fragrans, or “RiXiangGui”, is an ornamental, woody, evergreen plant that is cultivated widely because it blooms recurrently and emits a strong fragrance. Recently, the germplasm resources, classification, and aroma compositions of O. fragrans have been investigated. However, the molecular mechanisms of the floral scent formation and regulation have remained largely unknown. To obtain a global perspective on the molecular mechanism of the aroma formation during blooming, nine RNA Sequencing (RNA-Seq) libraries were constructed from three flowering stages: The initial, full, and final flowering stage. In short, a total of 523,961,310 high-quality clean reads were assembled into 136,611unigenes, with an average sequence length of 792 bp. About 47.43% of the unigenes (64,795) could be annotated in the NCBI non-redundant protein database. A number of candidate genes were identified in the terpenoid metabolic pathways and 1327 transcription factors (TFs), which showed differential expression patterns among the floral scent formation stages, were also identified, especially OfMYB1, OfMYB6, OfWRKY1, and OfWRKY3, which could play critical roles in the floral scent formation. These results indicated that the floral scent formation of O. fragrans was a very complex process which involved a large number of TFs. This study provides reliable resources for further studies of the O.fragrans floral scent formation. |
format | Online Article Text |
id | pubmed-6100529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61005292018-11-13 Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans Yang, Xiu-Lian Li, Hai-Yan Yue, Yuan-Zheng Ding, Wen-Jie Xu, Chen Shi, Ting-Ting Chen, Gong-Wei Wang, Liang-Gui Molecules Article Osmanthus fragrans, or “RiXiangGui”, is an ornamental, woody, evergreen plant that is cultivated widely because it blooms recurrently and emits a strong fragrance. Recently, the germplasm resources, classification, and aroma compositions of O. fragrans have been investigated. However, the molecular mechanisms of the floral scent formation and regulation have remained largely unknown. To obtain a global perspective on the molecular mechanism of the aroma formation during blooming, nine RNA Sequencing (RNA-Seq) libraries were constructed from three flowering stages: The initial, full, and final flowering stage. In short, a total of 523,961,310 high-quality clean reads were assembled into 136,611unigenes, with an average sequence length of 792 bp. About 47.43% of the unigenes (64,795) could be annotated in the NCBI non-redundant protein database. A number of candidate genes were identified in the terpenoid metabolic pathways and 1327 transcription factors (TFs), which showed differential expression patterns among the floral scent formation stages, were also identified, especially OfMYB1, OfMYB6, OfWRKY1, and OfWRKY3, which could play critical roles in the floral scent formation. These results indicated that the floral scent formation of O. fragrans was a very complex process which involved a large number of TFs. This study provides reliable resources for further studies of the O.fragrans floral scent formation. MDPI 2018-07-02 /pmc/articles/PMC6100529/ /pubmed/30004428 http://dx.doi.org/10.3390/molecules23071604 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Xiu-Lian Li, Hai-Yan Yue, Yuan-Zheng Ding, Wen-Jie Xu, Chen Shi, Ting-Ting Chen, Gong-Wei Wang, Liang-Gui Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title | Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title_full | Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title_fullStr | Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title_full_unstemmed | Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title_short | Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans |
title_sort | transcriptomic analysis of the candidate genes related to aroma formation in osmanthus fragrans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100529/ https://www.ncbi.nlm.nih.gov/pubmed/30004428 http://dx.doi.org/10.3390/molecules23071604 |
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