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Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice

Obesity is caused by an excess storage of body fat, resulting from a chronic imbalance between energy intake and expenditure. Gentiana lutea L. (GL) root has been reported to reduce lipid accumulation in the aortic wall of diabetic rats. Here, we performed fractionation and isolation of the bioactiv...

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Autores principales: Park, Eunkuk, Kim, Jeonghyun, Yeo, Subin, Kim, Gijeong, Ko, Eun-Hee, Lee, Sang Woo, Li, Wan Yi, Choi, Chun Whan, Jeong, Seon-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100558/
https://www.ncbi.nlm.nih.gov/pubmed/29987205
http://dx.doi.org/10.3390/molecules23071663
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author Park, Eunkuk
Kim, Jeonghyun
Yeo, Subin
Kim, Gijeong
Ko, Eun-Hee
Lee, Sang Woo
Li, Wan Yi
Choi, Chun Whan
Jeong, Seon-Yong
author_facet Park, Eunkuk
Kim, Jeonghyun
Yeo, Subin
Kim, Gijeong
Ko, Eun-Hee
Lee, Sang Woo
Li, Wan Yi
Choi, Chun Whan
Jeong, Seon-Yong
author_sort Park, Eunkuk
collection PubMed
description Obesity is caused by an excess storage of body fat, resulting from a chronic imbalance between energy intake and expenditure. Gentiana lutea L. (GL) root has been reported to reduce lipid accumulation in the aortic wall of diabetic rats. Here, we performed fractionation and isolation of the bioactive constituent(s) that may be responsible for the antiadipogenic effects of the GL root extract. A single compound, loganic acid, was identified as a candidate component in the 30% ethanol extract of GL. Loganic acid treatment significantly decreased the adipocyte differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. The expression of key adipogenesis-related genes such as adiponectin (Adipoq), peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), perilipin1 (Plin1), fatty acid binding protein 4 (Fabp4), glucose transporter type 4 (Slc2a4), CCAAT/enhancer-binding protein alpha (Cebpa), and tumor necrosis factor-alpha (Tnf) were significantly reduced following treatment with loganic acid. In vivo experiments in an ovariectomy-induced obesity mouse model showed that loganic acid (oral administration with 10 and 50 mg/kg/day) significantly inhibited body weight gain, total fat increase, fatty hepatocyte deposition in the liver, and adipocyte enlargement in the abdominal visceral fat tissues. These results suggest that loganic acid in the GL root extract has antiadipogenic effects in vitro and in vivo. Loganic acid may be beneficial for the prevention and treatment of obesity, particularly in menopausal obese women.
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spelling pubmed-61005582018-11-13 Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice Park, Eunkuk Kim, Jeonghyun Yeo, Subin Kim, Gijeong Ko, Eun-Hee Lee, Sang Woo Li, Wan Yi Choi, Chun Whan Jeong, Seon-Yong Molecules Article Obesity is caused by an excess storage of body fat, resulting from a chronic imbalance between energy intake and expenditure. Gentiana lutea L. (GL) root has been reported to reduce lipid accumulation in the aortic wall of diabetic rats. Here, we performed fractionation and isolation of the bioactive constituent(s) that may be responsible for the antiadipogenic effects of the GL root extract. A single compound, loganic acid, was identified as a candidate component in the 30% ethanol extract of GL. Loganic acid treatment significantly decreased the adipocyte differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. The expression of key adipogenesis-related genes such as adiponectin (Adipoq), peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), perilipin1 (Plin1), fatty acid binding protein 4 (Fabp4), glucose transporter type 4 (Slc2a4), CCAAT/enhancer-binding protein alpha (Cebpa), and tumor necrosis factor-alpha (Tnf) were significantly reduced following treatment with loganic acid. In vivo experiments in an ovariectomy-induced obesity mouse model showed that loganic acid (oral administration with 10 and 50 mg/kg/day) significantly inhibited body weight gain, total fat increase, fatty hepatocyte deposition in the liver, and adipocyte enlargement in the abdominal visceral fat tissues. These results suggest that loganic acid in the GL root extract has antiadipogenic effects in vitro and in vivo. Loganic acid may be beneficial for the prevention and treatment of obesity, particularly in menopausal obese women. MDPI 2018-07-09 /pmc/articles/PMC6100558/ /pubmed/29987205 http://dx.doi.org/10.3390/molecules23071663 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Eunkuk
Kim, Jeonghyun
Yeo, Subin
Kim, Gijeong
Ko, Eun-Hee
Lee, Sang Woo
Li, Wan Yi
Choi, Chun Whan
Jeong, Seon-Yong
Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title_full Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title_fullStr Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title_full_unstemmed Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title_short Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice
title_sort antiadipogenic effects of loganic acid in 3t3-l1 preadipocytes and ovariectomized mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100558/
https://www.ncbi.nlm.nih.gov/pubmed/29987205
http://dx.doi.org/10.3390/molecules23071663
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