Cargando…

Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA

The tuber of Cynanchum wilfordii (Baekshuoh Radix in Korean) is an important medicinal herb in Korea and China; however, it is difficult to differentiate C. wilfordii from a related medicinal herb, C. auriculatum (Baishouwu Radix in Chinese). We sought to develop a molecular method that could be use...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Yonguk, Choi, Hakjoon, Shin, Jawon, Jo, Ara, Lee, Kyung-Eun, Cho, Seung-Sik, Hwang, Yong-Pil, Choi, Chulyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100574/
https://www.ncbi.nlm.nih.gov/pubmed/29865213
http://dx.doi.org/10.3390/molecules23061337
_version_ 1783348906227138560
author Kim, Yonguk
Choi, Hakjoon
Shin, Jawon
Jo, Ara
Lee, Kyung-Eun
Cho, Seung-Sik
Hwang, Yong-Pil
Choi, Chulyung
author_facet Kim, Yonguk
Choi, Hakjoon
Shin, Jawon
Jo, Ara
Lee, Kyung-Eun
Cho, Seung-Sik
Hwang, Yong-Pil
Choi, Chulyung
author_sort Kim, Yonguk
collection PubMed
description The tuber of Cynanchum wilfordii (Baekshuoh Radix in Korean) is an important medicinal herb in Korea and China; however, it is difficult to differentiate C. wilfordii from a related medicinal herb, C. auriculatum (Baishouwu Radix in Chinese). We sought to develop a molecular method that could be used to distinguish between the tubers of C. wilfordii and C. auriculatum. We aligned the chloroplast genome sequences (available in the NCBI database) of the two species and identified three species-specific insertion and deletion (InDel) sites in the trnQ-psbK, rps2-rpoC2, and psaJ-rpl33 intergenic spacer (IGS) regions. To confirm the presence of these three InDels and validate their use as markers, we designed three primer pairs to amplify the trnQ-psbK, rps2-rpoC2, and psaJ-rpl33 IGS regions. Polymerase chain reaction (PCR) amplification of the trnQ-psbK IGS region yielded a 249 bp fragment for C. wilfordii, and 419 bp fragment for C. auriculatum, whereas the rps2-rpoC2 IGS primers produced a 629 bp fragment from C. wilfordii and a 282 bp fragment from C. auriculatum. In the psaJ-rpl33 IGS region, allele fragments of 342 and 360 bp in length were amplified from C. wilfordii, whereas 249 and 250 bp fragment were amplified from C. auriculatum. We propose these three InDel markers as a valuable, simple, and efficient tool for identifying these medicinal herbs and will thus reduce adulteration of these herbal materials in commercial markets.
format Online
Article
Text
id pubmed-6100574
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61005742018-11-13 Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA Kim, Yonguk Choi, Hakjoon Shin, Jawon Jo, Ara Lee, Kyung-Eun Cho, Seung-Sik Hwang, Yong-Pil Choi, Chulyung Molecules Technical Note The tuber of Cynanchum wilfordii (Baekshuoh Radix in Korean) is an important medicinal herb in Korea and China; however, it is difficult to differentiate C. wilfordii from a related medicinal herb, C. auriculatum (Baishouwu Radix in Chinese). We sought to develop a molecular method that could be used to distinguish between the tubers of C. wilfordii and C. auriculatum. We aligned the chloroplast genome sequences (available in the NCBI database) of the two species and identified three species-specific insertion and deletion (InDel) sites in the trnQ-psbK, rps2-rpoC2, and psaJ-rpl33 intergenic spacer (IGS) regions. To confirm the presence of these three InDels and validate their use as markers, we designed three primer pairs to amplify the trnQ-psbK, rps2-rpoC2, and psaJ-rpl33 IGS regions. Polymerase chain reaction (PCR) amplification of the trnQ-psbK IGS region yielded a 249 bp fragment for C. wilfordii, and 419 bp fragment for C. auriculatum, whereas the rps2-rpoC2 IGS primers produced a 629 bp fragment from C. wilfordii and a 282 bp fragment from C. auriculatum. In the psaJ-rpl33 IGS region, allele fragments of 342 and 360 bp in length were amplified from C. wilfordii, whereas 249 and 250 bp fragment were amplified from C. auriculatum. We propose these three InDel markers as a valuable, simple, and efficient tool for identifying these medicinal herbs and will thus reduce adulteration of these herbal materials in commercial markets. MDPI 2018-06-01 /pmc/articles/PMC6100574/ /pubmed/29865213 http://dx.doi.org/10.3390/molecules23061337 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Kim, Yonguk
Choi, Hakjoon
Shin, Jawon
Jo, Ara
Lee, Kyung-Eun
Cho, Seung-Sik
Hwang, Yong-Pil
Choi, Chulyung
Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title_full Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title_fullStr Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title_full_unstemmed Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title_short Molecular Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by InDel Markers of Chloroplast DNA
title_sort molecular discrimination of cynanchum wilfordii and cynanchum auriculatum by indel markers of chloroplast dna
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100574/
https://www.ncbi.nlm.nih.gov/pubmed/29865213
http://dx.doi.org/10.3390/molecules23061337
work_keys_str_mv AT kimyonguk moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT choihakjoon moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT shinjawon moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT joara moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT leekyungeun moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT choseungsik moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT hwangyongpil moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna
AT choichulyung moleculardiscriminationofcynanchumwilfordiiandcynanchumauriculatumbyindelmarkersofchloroplastdna