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De Novo Generation of Somatic Stem Cells by YAP/TAZ
Here we present protocols to isolate primary differentiated cells and turn them into stem/progenitor cells (SCs) of the same lineage by transient expression of the transcription factor YAP. With this method, luminal differentiated (LD) cells of the mouse mammary gland are converted into cells that e...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101120/ https://www.ncbi.nlm.nih.gov/pubmed/29782008 http://dx.doi.org/10.3791/57462 |
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author | Panciera, Tito Azzolin, Luca Di Biagio, Daniele Totaro, Antonio Cordenonsi, Michelangelo Piccolo, Stefano |
author_facet | Panciera, Tito Azzolin, Luca Di Biagio, Daniele Totaro, Antonio Cordenonsi, Michelangelo Piccolo, Stefano |
author_sort | Panciera, Tito |
collection | PubMed |
description | Here we present protocols to isolate primary differentiated cells and turn them into stem/progenitor cells (SCs) of the same lineage by transient expression of the transcription factor YAP. With this method, luminal differentiated (LD) cells of the mouse mammary gland are converted into cells that exhibit molecular and functional properties of mammary SCs. YAP also turns fully differentiated pancreatic exocrine cells into pancreatic duct-like progenitors. Similarly, to endogenous, natural SCs, YAP-induced stem-like cells ("ySCs") can be eventually expanded as organoid cultures long term in vitro, without further need of ectopic YAP/TAZ, as ySCs are endowed with a heritable self-renewing SC-like state. The reprogramming procedure presented here offers the possibility to generate and expand in vitro progenitor cells of various tissue sources starting from differentiated cells. The straightforward expansion of somatic cells ex vivo has implications for regenerative medicine, for understanding mechanisms of tumor initiation and, more in general, for cell and developmental biology studies. |
format | Online Article Text |
id | pubmed-6101120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-61011202018-09-06 De Novo Generation of Somatic Stem Cells by YAP/TAZ Panciera, Tito Azzolin, Luca Di Biagio, Daniele Totaro, Antonio Cordenonsi, Michelangelo Piccolo, Stefano J Vis Exp Biology Here we present protocols to isolate primary differentiated cells and turn them into stem/progenitor cells (SCs) of the same lineage by transient expression of the transcription factor YAP. With this method, luminal differentiated (LD) cells of the mouse mammary gland are converted into cells that exhibit molecular and functional properties of mammary SCs. YAP also turns fully differentiated pancreatic exocrine cells into pancreatic duct-like progenitors. Similarly, to endogenous, natural SCs, YAP-induced stem-like cells ("ySCs") can be eventually expanded as organoid cultures long term in vitro, without further need of ectopic YAP/TAZ, as ySCs are endowed with a heritable self-renewing SC-like state. The reprogramming procedure presented here offers the possibility to generate and expand in vitro progenitor cells of various tissue sources starting from differentiated cells. The straightforward expansion of somatic cells ex vivo has implications for regenerative medicine, for understanding mechanisms of tumor initiation and, more in general, for cell and developmental biology studies. MyJove Corporation 2018-05-07 /pmc/articles/PMC6101120/ /pubmed/29782008 http://dx.doi.org/10.3791/57462 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Biology Panciera, Tito Azzolin, Luca Di Biagio, Daniele Totaro, Antonio Cordenonsi, Michelangelo Piccolo, Stefano De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title | De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title_full | De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title_fullStr | De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title_full_unstemmed | De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title_short | De Novo Generation of Somatic Stem Cells by YAP/TAZ |
title_sort | de novo generation of somatic stem cells by yap/taz |
topic | Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101120/ https://www.ncbi.nlm.nih.gov/pubmed/29782008 http://dx.doi.org/10.3791/57462 |
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