Solid phase chemistry to covalently and reversibly capture thiolated RNA

Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s(4)U) metabolic labeling and solid phase chemistry. This one-step enrichment procedure captures s(4)U-RNA by using highly efficient methane thiosulfonate (MTS) chemistry in an immobilized...

Descripción completa

Detalles Bibliográficos
Autores principales: Duffy, Erin E, Canzio, Daniele, Maniatis, Tom, Simon, Matthew D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101502/
https://www.ncbi.nlm.nih.gov/pubmed/29986098
http://dx.doi.org/10.1093/nar/gky556

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101502/
https://www.ncbi.nlm.nih.gov/pubmed/29986098
http://dx.doi.org/10.1093/nar/gky556

Ejemplares similares