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Solid phase chemistry to covalently and reversibly capture thiolated RNA

Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s(4)U) metabolic labeling and solid phase chemistry. This one-step enrichment procedure captures s(4)U-RNA by using highly efficient methane thiosulfonate (MTS) chemistry in an immobilized...

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Detalles Bibliográficos
Autores principales:	Duffy, Erin E, Canzio, Daniele, Maniatis, Tom, Simon, Matthew D
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101502/ https://www.ncbi.nlm.nih.gov/pubmed/29986098 http://dx.doi.org/10.1093/nar/gky556

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