A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells

Base editing is an exciting new genome engineering technology. C-to-T mutations in genomic DNA have been achieved using ribonucleoprotein complexes comprised of rat APOBEC1 single-stranded DNA deaminase, Cas9 nickase (Cas9n), uracil DNA glycosylase inhibitor (UGI), and guide (g)RNA. Here, we report...

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Detalles Bibliográficos
Autores principales: St. Martin, Amber, Salamango, Daniel, Serebrenik, Artur, Shaban, Nadine, Brown, William L, Donati, Francesco, Munagala, Uday, Conticello, Silvestro G, Harris, Reuben S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101615/
https://www.ncbi.nlm.nih.gov/pubmed/29746667
http://dx.doi.org/10.1093/nar/gky332

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