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PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke
Neuroinflammation is central to the pathological cascade following ischemic stroke. Non-invasive molecular imaging methods have the potential to provide critical insights into the temporal dynamics and role of certain neuroimmune interactions in stroke. Specifically, Positron Emission Tomography (PE...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101726/ https://www.ncbi.nlm.nih.gov/pubmed/29985311 http://dx.doi.org/10.3791/57243 |
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author | Chaney, Aisling M. Johnson, Emily M. Cropper, Haley C. James, Michelle L. |
author_facet | Chaney, Aisling M. Johnson, Emily M. Cropper, Haley C. James, Michelle L. |
author_sort | Chaney, Aisling M. |
collection | PubMed |
description | Neuroinflammation is central to the pathological cascade following ischemic stroke. Non-invasive molecular imaging methods have the potential to provide critical insights into the temporal dynamics and role of certain neuroimmune interactions in stroke. Specifically, Positron Emission Tomography (PET) imaging of translocator protein 18 kDa (TSPO), a marker of activated microglia and peripheral myeloid-lineage cells, provides a means to detect and track neuroinflammation in vivo. Here, we present a method to accurately quantify neuroinflammation using [(11)C]N,N-Diethyl-2-[2-(4-methoxyphenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl]acetamide ([(11)C]DPA-713), a promising second generation TSPO-PET radiotracer, in distal middle cerebral artery occlusion (dMCAO) compared to sham-operated mice. MRI was performed 2 days post-dMCAO surgery to confirm stroke and define the infarct location and volume. PET/Computed Tomography (CT) imaging was carried out 6 days post-dMCAO to capture the peak increase in TSPO levels following stroke. Quantitation of PET images was conducted to assess the uptake of [(11)C]DPA-713 in the brain and spleen of dMCAO and sham mice to assess central and peripheral levels of inflammation. In vivo [(11)C]DPA-713 brain uptake was confirmed using ex vivo autoradiography. |
format | Online Article Text |
id | pubmed-6101726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-61017262018-09-11 PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke Chaney, Aisling M. Johnson, Emily M. Cropper, Haley C. James, Michelle L. J Vis Exp Medicine Neuroinflammation is central to the pathological cascade following ischemic stroke. Non-invasive molecular imaging methods have the potential to provide critical insights into the temporal dynamics and role of certain neuroimmune interactions in stroke. Specifically, Positron Emission Tomography (PET) imaging of translocator protein 18 kDa (TSPO), a marker of activated microglia and peripheral myeloid-lineage cells, provides a means to detect and track neuroinflammation in vivo. Here, we present a method to accurately quantify neuroinflammation using [(11)C]N,N-Diethyl-2-[2-(4-methoxyphenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl]acetamide ([(11)C]DPA-713), a promising second generation TSPO-PET radiotracer, in distal middle cerebral artery occlusion (dMCAO) compared to sham-operated mice. MRI was performed 2 days post-dMCAO surgery to confirm stroke and define the infarct location and volume. PET/Computed Tomography (CT) imaging was carried out 6 days post-dMCAO to capture the peak increase in TSPO levels following stroke. Quantitation of PET images was conducted to assess the uptake of [(11)C]DPA-713 in the brain and spleen of dMCAO and sham mice to assess central and peripheral levels of inflammation. In vivo [(11)C]DPA-713 brain uptake was confirmed using ex vivo autoradiography. MyJove Corporation 2018-06-14 /pmc/articles/PMC6101726/ /pubmed/29985311 http://dx.doi.org/10.3791/57243 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Medicine Chaney, Aisling M. Johnson, Emily M. Cropper, Haley C. James, Michelle L. PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title | PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title_full | PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title_fullStr | PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title_full_unstemmed | PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title_short | PET Imaging of Neuroinflammation Using [(11)C]DPA-713 in a Mouse Model of Ischemic Stroke |
title_sort | pet imaging of neuroinflammation using [(11)c]dpa-713 in a mouse model of ischemic stroke |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101726/ https://www.ncbi.nlm.nih.gov/pubmed/29985311 http://dx.doi.org/10.3791/57243 |
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