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Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

In this protocol, we present methods to fabricate thin elastomer composite films for advanced cell culture applications and for the development of skin adhesives. Two different poly-(dimethyl siloxanes) (PDMS and soft skin adhesive (SSA)), have been used for in depth investigation of biological effe...

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Autores principales: Boyadzhieva, Silviya, Fischer, Sarah C.L., Lösch, Svenja, Rutz, Angela, Arzt, Eduard, Kruttwig, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102035/
https://www.ncbi.nlm.nih.gov/pubmed/30035765
http://dx.doi.org/10.3791/57573
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author Boyadzhieva, Silviya
Fischer, Sarah C.L.
Lösch, Svenja
Rutz, Angela
Arzt, Eduard
Kruttwig, Klaus
author_facet Boyadzhieva, Silviya
Fischer, Sarah C.L.
Lösch, Svenja
Rutz, Angela
Arzt, Eduard
Kruttwig, Klaus
author_sort Boyadzhieva, Silviya
collection PubMed
description In this protocol, we present methods to fabricate thin elastomer composite films for advanced cell culture applications and for the development of skin adhesives. Two different poly-(dimethyl siloxanes) (PDMS and soft skin adhesive (SSA)), have been used for in depth investigation of biological effects and adhesive characteristics. The composite films consist of a flexible backing layer and an adhesive top coating. Both layers have been manufactured by doctor blade application technique. In the present investigation, the adhesive behavior of the composite films has been investigated as a function of the layer thickness or a variation of the Young's modulus of the top layer. The Young's modulus of PDMS has been changed by varying the base to crosslinker mixing ratio. In addition, the thickness of SSA films has been varied from approx. 16 µm to approx. 320 µm. Scanning electron microscopy (SEM) and optical microscopy have been used for thickness measurements. The adhesive properties of elastomer films depend strongly on the film thickness, the Young's modulus of the polymers and surface characteristics. Therefore, normal adhesion of these films on glass substrates exhibiting smooth and rough surfaces has been investigated. Pull-off stress and work of separation are dependent on the mixing ratio of silicone elastomers. Additionally, the thickness of the soft skin adhesive placed on top of a supportive backing layer has been varied in order to produce patches for skin applications. Cytotoxicity, proliferation and cellular adhesion of L929 murine fibroblasts on PDMS films (mixing ratio 10:1) and SSA films (mixing ratio 50:50) have been conducted. We have shown here, for the first time, the side by side comparison of thin composite films manufactured of both polymers and present the investigation of their biological- and adhesive properties.
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spelling pubmed-61020352018-09-05 Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization Boyadzhieva, Silviya Fischer, Sarah C.L. Lösch, Svenja Rutz, Angela Arzt, Eduard Kruttwig, Klaus J Vis Exp Developmental Biology In this protocol, we present methods to fabricate thin elastomer composite films for advanced cell culture applications and for the development of skin adhesives. Two different poly-(dimethyl siloxanes) (PDMS and soft skin adhesive (SSA)), have been used for in depth investigation of biological effects and adhesive characteristics. The composite films consist of a flexible backing layer and an adhesive top coating. Both layers have been manufactured by doctor blade application technique. In the present investigation, the adhesive behavior of the composite films has been investigated as a function of the layer thickness or a variation of the Young's modulus of the top layer. The Young's modulus of PDMS has been changed by varying the base to crosslinker mixing ratio. In addition, the thickness of SSA films has been varied from approx. 16 µm to approx. 320 µm. Scanning electron microscopy (SEM) and optical microscopy have been used for thickness measurements. The adhesive properties of elastomer films depend strongly on the film thickness, the Young's modulus of the polymers and surface characteristics. Therefore, normal adhesion of these films on glass substrates exhibiting smooth and rough surfaces has been investigated. Pull-off stress and work of separation are dependent on the mixing ratio of silicone elastomers. Additionally, the thickness of the soft skin adhesive placed on top of a supportive backing layer has been varied in order to produce patches for skin applications. Cytotoxicity, proliferation and cellular adhesion of L929 murine fibroblasts on PDMS films (mixing ratio 10:1) and SSA films (mixing ratio 50:50) have been conducted. We have shown here, for the first time, the side by side comparison of thin composite films manufactured of both polymers and present the investigation of their biological- and adhesive properties. MyJove Corporation 2018-07-03 /pmc/articles/PMC6102035/ /pubmed/30035765 http://dx.doi.org/10.3791/57573 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Developmental Biology
Boyadzhieva, Silviya
Fischer, Sarah C.L.
Lösch, Svenja
Rutz, Angela
Arzt, Eduard
Kruttwig, Klaus
Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title_full Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title_fullStr Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title_full_unstemmed Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title_short Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
title_sort thin film composite silicon elastomers for cell culture and skin applications: manufacturing and characterization
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102035/
https://www.ncbi.nlm.nih.gov/pubmed/30035765
http://dx.doi.org/10.3791/57573
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