Cargando…

Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions

Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio...

Descripción completa

Detalles Bibliográficos
Autores principales: Moloudi, Reza, Oh, Steve, Yang, Chun, Teo, Kim Leng, Lam, Alan Tin-Lun, Warkiani, Majid Ebrahimi, Naing, May Win
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102204/
https://www.ncbi.nlm.nih.gov/pubmed/30127526
http://dx.doi.org/10.1038/s41598-018-31019-y
_version_ 1783349108699824128
author Moloudi, Reza
Oh, Steve
Yang, Chun
Teo, Kim Leng
Lam, Alan Tin-Lun
Warkiani, Majid Ebrahimi
Naing, May Win
author_facet Moloudi, Reza
Oh, Steve
Yang, Chun
Teo, Kim Leng
Lam, Alan Tin-Lun
Warkiani, Majid Ebrahimi
Naing, May Win
author_sort Moloudi, Reza
collection PubMed
description Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5).
format Online
Article
Text
id pubmed-6102204
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61022042018-08-27 Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions Moloudi, Reza Oh, Steve Yang, Chun Teo, Kim Leng Lam, Alan Tin-Lun Warkiani, Majid Ebrahimi Naing, May Win Sci Rep Article Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α) = 0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5). Nature Publishing Group UK 2018-08-20 /pmc/articles/PMC6102204/ /pubmed/30127526 http://dx.doi.org/10.1038/s41598-018-31019-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moloudi, Reza
Oh, Steve
Yang, Chun
Teo, Kim Leng
Lam, Alan Tin-Lun
Warkiani, Majid Ebrahimi
Naing, May Win
Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title_full Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title_fullStr Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title_full_unstemmed Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title_short Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions
title_sort inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102204/
https://www.ncbi.nlm.nih.gov/pubmed/30127526
http://dx.doi.org/10.1038/s41598-018-31019-y
work_keys_str_mv AT moloudireza inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT ohsteve inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT yangchun inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT teokimleng inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT lamalantinlun inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT warkianimajidebrahimi inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions
AT naingmaywin inertialbasedfiltrationmethodforremovalofmicrocarriersfrommesenchymalstemcellsuspensions