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Towards standardized mechanical characterization of microbial biofilms: analysis and critical review
Developing reliable anti-biofilm strategies or efficient biofilm-based bioprocesses strongly depends on having a clear understanding of the mechanisms underlying biofilm development, and knowledge of the relevant mechanical parameters describing microbial biofilm behavior. Many varied mechanical tes...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102240/ https://www.ncbi.nlm.nih.gov/pubmed/30131867 http://dx.doi.org/10.1038/s41522-018-0062-5 |
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author | Boudarel, Héloïse Mathias, Jean-Denis Blaysat, Benoît Grédiac, Michel |
author_facet | Boudarel, Héloïse Mathias, Jean-Denis Blaysat, Benoît Grédiac, Michel |
author_sort | Boudarel, Héloïse |
collection | PubMed |
description | Developing reliable anti-biofilm strategies or efficient biofilm-based bioprocesses strongly depends on having a clear understanding of the mechanisms underlying biofilm development, and knowledge of the relevant mechanical parameters describing microbial biofilm behavior. Many varied mechanical testing methods are available to assess these parameters. The mechanical properties thus identified can then be used to compare protocols such as antibiotic screening. However, the lack of standardization in both mechanical testing and the associated identification methods for a given microbiological goal remains a blind spot in the biofilm community. The pursuit of standardization is problematic, as biofilms are living structures, i.e., both complex and dynamic. Here, we review the main available methods for characterizing the mechanical properties of biofilms through the lens of the relationship linking experimental testing to the identification of mechanical parameters. We propose guidelines for characterizing biofilms according to microbiological objectives that will help the reader choose an appropriate test and a relevant identification method for measuring any given mechanical parameter. The use of a common methodology for the mechanical characterization of biofilms will enable reliable analysis and comparison of microbiological protocols needed for improvement of engineering process and screening. |
format | Online Article Text |
id | pubmed-6102240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61022402018-08-21 Towards standardized mechanical characterization of microbial biofilms: analysis and critical review Boudarel, Héloïse Mathias, Jean-Denis Blaysat, Benoît Grédiac, Michel NPJ Biofilms Microbiomes Review Article Developing reliable anti-biofilm strategies or efficient biofilm-based bioprocesses strongly depends on having a clear understanding of the mechanisms underlying biofilm development, and knowledge of the relevant mechanical parameters describing microbial biofilm behavior. Many varied mechanical testing methods are available to assess these parameters. The mechanical properties thus identified can then be used to compare protocols such as antibiotic screening. However, the lack of standardization in both mechanical testing and the associated identification methods for a given microbiological goal remains a blind spot in the biofilm community. The pursuit of standardization is problematic, as biofilms are living structures, i.e., both complex and dynamic. Here, we review the main available methods for characterizing the mechanical properties of biofilms through the lens of the relationship linking experimental testing to the identification of mechanical parameters. We propose guidelines for characterizing biofilms according to microbiological objectives that will help the reader choose an appropriate test and a relevant identification method for measuring any given mechanical parameter. The use of a common methodology for the mechanical characterization of biofilms will enable reliable analysis and comparison of microbiological protocols needed for improvement of engineering process and screening. Nature Publishing Group UK 2018-08-20 /pmc/articles/PMC6102240/ /pubmed/30131867 http://dx.doi.org/10.1038/s41522-018-0062-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Article Boudarel, Héloïse Mathias, Jean-Denis Blaysat, Benoît Grédiac, Michel Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title | Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title_full | Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title_fullStr | Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title_full_unstemmed | Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title_short | Towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
title_sort | towards standardized mechanical characterization of microbial biofilms: analysis and critical review |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102240/ https://www.ncbi.nlm.nih.gov/pubmed/30131867 http://dx.doi.org/10.1038/s41522-018-0062-5 |
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