Iridium-based probe for luminescent nitric oxide monitoring in live cells

Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 display...

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Autores principales: Wu, Chun, Wu, Ke-Jia, Kang, Tian-Shu, Wang, Hui-Min David, Leung, Chung-Hang, Liu, Jin-Biao, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102254/
https://www.ncbi.nlm.nih.gov/pubmed/30127525
http://dx.doi.org/10.1038/s41598-018-30991-9
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author Wu, Chun
Wu, Ke-Jia
Kang, Tian-Shu
Wang, Hui-Min David
Leung, Chung-Hang
Liu, Jin-Biao
Ma, Dik-Lung
author_facet Wu, Chun
Wu, Ke-Jia
Kang, Tian-Shu
Wang, Hui-Min David
Leung, Chung-Hang
Liu, Jin-Biao
Ma, Dik-Lung
author_sort Wu, Chun
collection PubMed
description Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.
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spelling pubmed-61022542018-08-27 Iridium-based probe for luminescent nitric oxide monitoring in live cells Wu, Chun Wu, Ke-Jia Kang, Tian-Shu Wang, Hui-Min David Leung, Chung-Hang Liu, Jin-Biao Ma, Dik-Lung Sci Rep Article Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment. Nature Publishing Group UK 2018-08-20 /pmc/articles/PMC6102254/ /pubmed/30127525 http://dx.doi.org/10.1038/s41598-018-30991-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wu, Chun
Wu, Ke-Jia
Kang, Tian-Shu
Wang, Hui-Min David
Leung, Chung-Hang
Liu, Jin-Biao
Ma, Dik-Lung
Iridium-based probe for luminescent nitric oxide monitoring in live cells
title Iridium-based probe for luminescent nitric oxide monitoring in live cells
title_full Iridium-based probe for luminescent nitric oxide monitoring in live cells
title_fullStr Iridium-based probe for luminescent nitric oxide monitoring in live cells
title_full_unstemmed Iridium-based probe for luminescent nitric oxide monitoring in live cells
title_short Iridium-based probe for luminescent nitric oxide monitoring in live cells
title_sort iridium-based probe for luminescent nitric oxide monitoring in live cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102254/
https://www.ncbi.nlm.nih.gov/pubmed/30127525
http://dx.doi.org/10.1038/s41598-018-30991-9
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