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A Chemical Investigation of the Leaves of Morus alba L.
The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute config...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102566/ https://www.ncbi.nlm.nih.gov/pubmed/29701695 http://dx.doi.org/10.3390/molecules23051018 |
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author | Chen, Xiao-yan Zhang, Ting Wang, Xin Hamann, Mark T. Kang, Jie Yu, De-quan Chen, Ruo-yun |
author_facet | Chen, Xiao-yan Zhang, Ting Wang, Xin Hamann, Mark T. Kang, Jie Yu, De-quan Chen, Ruo-yun |
author_sort | Chen, Xiao-yan |
collection | PubMed |
description | The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1–4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC(50) values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC(50) 1.88 × 10(−3) μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001). |
format | Online Article Text |
id | pubmed-6102566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61025662018-11-13 A Chemical Investigation of the Leaves of Morus alba L. Chen, Xiao-yan Zhang, Ting Wang, Xin Hamann, Mark T. Kang, Jie Yu, De-quan Chen, Ruo-yun Molecules Article The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1–4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC(50) values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC(50) 1.88 × 10(−3) μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001). MDPI 2018-04-26 /pmc/articles/PMC6102566/ /pubmed/29701695 http://dx.doi.org/10.3390/molecules23051018 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Xiao-yan Zhang, Ting Wang, Xin Hamann, Mark T. Kang, Jie Yu, De-quan Chen, Ruo-yun A Chemical Investigation of the Leaves of Morus alba L. |
title | A Chemical Investigation of the Leaves of Morus alba L. |
title_full | A Chemical Investigation of the Leaves of Morus alba L. |
title_fullStr | A Chemical Investigation of the Leaves of Morus alba L. |
title_full_unstemmed | A Chemical Investigation of the Leaves of Morus alba L. |
title_short | A Chemical Investigation of the Leaves of Morus alba L. |
title_sort | chemical investigation of the leaves of morus alba l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102566/ https://www.ncbi.nlm.nih.gov/pubmed/29701695 http://dx.doi.org/10.3390/molecules23051018 |
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