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A Chemical Investigation of the Leaves of Morus alba L.

The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute config...

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Autores principales: Chen, Xiao-yan, Zhang, Ting, Wang, Xin, Hamann, Mark T., Kang, Jie, Yu, De-quan, Chen, Ruo-yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102566/
https://www.ncbi.nlm.nih.gov/pubmed/29701695
http://dx.doi.org/10.3390/molecules23051018
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author Chen, Xiao-yan
Zhang, Ting
Wang, Xin
Hamann, Mark T.
Kang, Jie
Yu, De-quan
Chen, Ruo-yun
author_facet Chen, Xiao-yan
Zhang, Ting
Wang, Xin
Hamann, Mark T.
Kang, Jie
Yu, De-quan
Chen, Ruo-yun
author_sort Chen, Xiao-yan
collection PubMed
description The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1–4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC(50) values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC(50) 1.88 × 10(−3) μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001).
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spelling pubmed-61025662018-11-13 A Chemical Investigation of the Leaves of Morus alba L. Chen, Xiao-yan Zhang, Ting Wang, Xin Hamann, Mark T. Kang, Jie Yu, De-quan Chen, Ruo-yun Molecules Article The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1–4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC(50) values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC(50) 1.88 × 10(−3) μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001). MDPI 2018-04-26 /pmc/articles/PMC6102566/ /pubmed/29701695 http://dx.doi.org/10.3390/molecules23051018 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Xiao-yan
Zhang, Ting
Wang, Xin
Hamann, Mark T.
Kang, Jie
Yu, De-quan
Chen, Ruo-yun
A Chemical Investigation of the Leaves of Morus alba L.
title A Chemical Investigation of the Leaves of Morus alba L.
title_full A Chemical Investigation of the Leaves of Morus alba L.
title_fullStr A Chemical Investigation of the Leaves of Morus alba L.
title_full_unstemmed A Chemical Investigation of the Leaves of Morus alba L.
title_short A Chemical Investigation of the Leaves of Morus alba L.
title_sort chemical investigation of the leaves of morus alba l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102566/
https://www.ncbi.nlm.nih.gov/pubmed/29701695
http://dx.doi.org/10.3390/molecules23051018
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