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Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species

Aconitum (Ranunculaceae) consists of approximately 400 species distributed in the temperate regions of the northern hemisphere. Many species are well-known herbs, mainly used for analgesia and anti-inflammatory purposes. This genus is well represented in China and has gained widespread attention for...

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Autores principales: Meng, Jing, Li, Xuepei, Li, Hongtao, Yang, Junbo, Wang, Hong, He, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102581/
https://www.ncbi.nlm.nih.gov/pubmed/29701675
http://dx.doi.org/10.3390/molecules23051015
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author Meng, Jing
Li, Xuepei
Li, Hongtao
Yang, Junbo
Wang, Hong
He, Jun
author_facet Meng, Jing
Li, Xuepei
Li, Hongtao
Yang, Junbo
Wang, Hong
He, Jun
author_sort Meng, Jing
collection PubMed
description Aconitum (Ranunculaceae) consists of approximately 400 species distributed in the temperate regions of the northern hemisphere. Many species are well-known herbs, mainly used for analgesia and anti-inflammatory purposes. This genus is well represented in China and has gained widespread attention for its toxicity and detoxification properties. In southwestern China, several Aconitum species, called ‘Dula’ in the Yi Nationality, were often used to control the poisonous effects of other Aconitum plants. In this study, the complete chloroplast (cp) genomes of these species were determined for the first time through Illumina paired-end sequencing. Our results indicate that their cp genomes ranged from 151,214 bp (A. episcopale) to 155,769 bp (A. delavayi) in length. A total of 111–112 unique genes were identified, including 85 protein-coding genes, 36–37 tRNA genes and eight ribosomal RNA genes (rRNA). We also analyzed codon usage, IR expansion or contraction and simple sequence repeats in the cp genomes. Eight variable regions were identified and these may potentially be useful as specific DNA barcodes for species identification of Aconitum. Phylogenetic analysis revealed that all five studied species formed a new clade and were resolved with 100% bootstrap support. This study will provide genomic resources and potential plastid markers for DNA barcoding, further taxonomy and germplasm exploration of Aconitum.
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spelling pubmed-61025812018-11-13 Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species Meng, Jing Li, Xuepei Li, Hongtao Yang, Junbo Wang, Hong He, Jun Molecules Article Aconitum (Ranunculaceae) consists of approximately 400 species distributed in the temperate regions of the northern hemisphere. Many species are well-known herbs, mainly used for analgesia and anti-inflammatory purposes. This genus is well represented in China and has gained widespread attention for its toxicity and detoxification properties. In southwestern China, several Aconitum species, called ‘Dula’ in the Yi Nationality, were often used to control the poisonous effects of other Aconitum plants. In this study, the complete chloroplast (cp) genomes of these species were determined for the first time through Illumina paired-end sequencing. Our results indicate that their cp genomes ranged from 151,214 bp (A. episcopale) to 155,769 bp (A. delavayi) in length. A total of 111–112 unique genes were identified, including 85 protein-coding genes, 36–37 tRNA genes and eight ribosomal RNA genes (rRNA). We also analyzed codon usage, IR expansion or contraction and simple sequence repeats in the cp genomes. Eight variable regions were identified and these may potentially be useful as specific DNA barcodes for species identification of Aconitum. Phylogenetic analysis revealed that all five studied species formed a new clade and were resolved with 100% bootstrap support. This study will provide genomic resources and potential plastid markers for DNA barcoding, further taxonomy and germplasm exploration of Aconitum. MDPI 2018-04-26 /pmc/articles/PMC6102581/ /pubmed/29701675 http://dx.doi.org/10.3390/molecules23051015 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meng, Jing
Li, Xuepei
Li, Hongtao
Yang, Junbo
Wang, Hong
He, Jun
Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title_full Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title_fullStr Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title_full_unstemmed Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title_short Comparative Analysis of the Complete Chloroplast Genomes of Four Aconitum Medicinal Species
title_sort comparative analysis of the complete chloroplast genomes of four aconitum medicinal species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102581/
https://www.ncbi.nlm.nih.gov/pubmed/29701675
http://dx.doi.org/10.3390/molecules23051015
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