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Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa
Bioassay-guided fractionation of the chloroform extract of Tapinella atrotomentosa led to the isolation of four secondary metabolites 1–4. Two of the compounds are lactones—osmundalactone (1) and 5-hydroxy-hex-2-en-4-olide (2)—while 3 and 4 were identified as terphenyl quinones, spiromentins C and B...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102582/ https://www.ncbi.nlm.nih.gov/pubmed/29734648 http://dx.doi.org/10.3390/molecules23051082 |
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author | Béni, Zoltán Dékány, Miklós Kovács, Bernadett Csupor-Löffler, Boglárka Zomborszki, Zoltán Péter Kerekes, Erika Szekeres, András Urbán, Edit Hohmann, Judit Ványolós, Attila |
author_facet | Béni, Zoltán Dékány, Miklós Kovács, Bernadett Csupor-Löffler, Boglárka Zomborszki, Zoltán Péter Kerekes, Erika Szekeres, András Urbán, Edit Hohmann, Judit Ványolós, Attila |
author_sort | Béni, Zoltán |
collection | PubMed |
description | Bioassay-guided fractionation of the chloroform extract of Tapinella atrotomentosa led to the isolation of four secondary metabolites 1–4. Two of the compounds are lactones—osmundalactone (1) and 5-hydroxy-hex-2-en-4-olide (2)—while 3 and 4 were identified as terphenyl quinones, spiromentins C and B, respectively. The structures of the compounds were established on the basis of NMR and MS spectroscopic analysis. The isolated fungal metabolites were evaluated for their antibacterial activities against several Gram-positive and negative bacteria. In addition, their synergistic effect with cefuroxime against methicillin-resistant Staphylococcus aureus (MRSA) was also evaluated. Compounds 1–3 proved to possess significant antibacterial activity against multiresistant Acinetobacter baumannii and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The investigation of the antioxidant effect of the isolated compounds in DPPH and ORAC assays revealed that spiromentins C (3) and B (4) have remarkable antioxidant activity. |
format | Online Article Text |
id | pubmed-6102582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61025822018-11-13 Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa Béni, Zoltán Dékány, Miklós Kovács, Bernadett Csupor-Löffler, Boglárka Zomborszki, Zoltán Péter Kerekes, Erika Szekeres, András Urbán, Edit Hohmann, Judit Ványolós, Attila Molecules Article Bioassay-guided fractionation of the chloroform extract of Tapinella atrotomentosa led to the isolation of four secondary metabolites 1–4. Two of the compounds are lactones—osmundalactone (1) and 5-hydroxy-hex-2-en-4-olide (2)—while 3 and 4 were identified as terphenyl quinones, spiromentins C and B, respectively. The structures of the compounds were established on the basis of NMR and MS spectroscopic analysis. The isolated fungal metabolites were evaluated for their antibacterial activities against several Gram-positive and negative bacteria. In addition, their synergistic effect with cefuroxime against methicillin-resistant Staphylococcus aureus (MRSA) was also evaluated. Compounds 1–3 proved to possess significant antibacterial activity against multiresistant Acinetobacter baumannii and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The investigation of the antioxidant effect of the isolated compounds in DPPH and ORAC assays revealed that spiromentins C (3) and B (4) have remarkable antioxidant activity. MDPI 2018-05-04 /pmc/articles/PMC6102582/ /pubmed/29734648 http://dx.doi.org/10.3390/molecules23051082 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Béni, Zoltán Dékány, Miklós Kovács, Bernadett Csupor-Löffler, Boglárka Zomborszki, Zoltán Péter Kerekes, Erika Szekeres, András Urbán, Edit Hohmann, Judit Ványolós, Attila Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title | Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title_full | Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title_fullStr | Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title_full_unstemmed | Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title_short | Bioactivity-Guided Isolation of Antimicrobial and Antioxidant Metabolites from the Mushroom Tapinella atrotomentosa |
title_sort | bioactivity-guided isolation of antimicrobial and antioxidant metabolites from the mushroom tapinella atrotomentosa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102582/ https://www.ncbi.nlm.nih.gov/pubmed/29734648 http://dx.doi.org/10.3390/molecules23051082 |
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