Cargando…
Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction
Obesity may induce end-organ damage through metabolic syndrome, and autophagy serves a vital role in the pathogenesis of metabolic syndrome. The purpose of the present study was to define the roles of autophagy and mitophagy in high fat diet (HFD)-induced cardiomyopathy. Male, 8 week-old C57BL/6 mic...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102660/ https://www.ncbi.nlm.nih.gov/pubmed/30066870 http://dx.doi.org/10.3892/mmr.2018.9301 |
_version_ | 1783349215195299840 |
---|---|
author | Che, Yan Wang, Zhao-Peng Yuan, Yuan Zhang, Ning Jin, Ya-Ge Wan, Chun-Xia Tang, Qi-Zhu |
author_facet | Che, Yan Wang, Zhao-Peng Yuan, Yuan Zhang, Ning Jin, Ya-Ge Wan, Chun-Xia Tang, Qi-Zhu |
author_sort | Che, Yan |
collection | PubMed |
description | Obesity may induce end-organ damage through metabolic syndrome, and autophagy serves a vital role in the pathogenesis of metabolic syndrome. The purpose of the present study was to define the roles of autophagy and mitophagy in high fat diet (HFD)-induced cardiomyopathy. Male, 8 week-old C57BL/6 mice were fed either a HFD (60% kcal) or a diet of normal chow (NC; 10% kcal) for 42 weeks. Glucose tolerance tests were performed during the feeding regimes. Blood samples were collected for assaying serum triglyceride with the glycerol-3-phosphate oxidase phenol and aminophenazone (PAP) method and total cholesterol was tested with the cholesterol oxidase-PAP method. Myocardial function was assessed using echocardiography and hemodynamic analyses. Western blot analysis was employed to evaluate endoplasmic reticulum stress (ERS), autophagy and mitochondrial function. Electron microscopy was used to assess the number of lipid droplets and the degree of autophagy within the myocardium. The body weight and adipose tissue weight of mice fed the HFD were increased compared with the NC mice. The serum levels of blood glucose, total cholesterol and triglyceride were significantly increased following 42 weeks of HFD feeding. The results of the glucose tolerance tests additionally demonstrated metabolic dysregulation in HFD mice. In addition, HFD mice exhibited hemodynamic and echocardiographic evidence of impaired diastolic and systolic function, including alterations in the cardiac output, end-diastolic pressure, end-diastolic volume and left ventricular relaxation time constant (tau) following HFD intake. Furthermore, a HFD resulted in increased ERS, and a downregulation of the autophagy and mitophagy level. The present study investigated cardiac function in obese HFD-fed mice. These results aid the pursuit of novel therapeutic targets to combat obesity-associated cardiomyopathy. |
format | Online Article Text |
id | pubmed-6102660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61026602018-08-23 Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction Che, Yan Wang, Zhao-Peng Yuan, Yuan Zhang, Ning Jin, Ya-Ge Wan, Chun-Xia Tang, Qi-Zhu Mol Med Rep Articles Obesity may induce end-organ damage through metabolic syndrome, and autophagy serves a vital role in the pathogenesis of metabolic syndrome. The purpose of the present study was to define the roles of autophagy and mitophagy in high fat diet (HFD)-induced cardiomyopathy. Male, 8 week-old C57BL/6 mice were fed either a HFD (60% kcal) or a diet of normal chow (NC; 10% kcal) for 42 weeks. Glucose tolerance tests were performed during the feeding regimes. Blood samples were collected for assaying serum triglyceride with the glycerol-3-phosphate oxidase phenol and aminophenazone (PAP) method and total cholesterol was tested with the cholesterol oxidase-PAP method. Myocardial function was assessed using echocardiography and hemodynamic analyses. Western blot analysis was employed to evaluate endoplasmic reticulum stress (ERS), autophagy and mitochondrial function. Electron microscopy was used to assess the number of lipid droplets and the degree of autophagy within the myocardium. The body weight and adipose tissue weight of mice fed the HFD were increased compared with the NC mice. The serum levels of blood glucose, total cholesterol and triglyceride were significantly increased following 42 weeks of HFD feeding. The results of the glucose tolerance tests additionally demonstrated metabolic dysregulation in HFD mice. In addition, HFD mice exhibited hemodynamic and echocardiographic evidence of impaired diastolic and systolic function, including alterations in the cardiac output, end-diastolic pressure, end-diastolic volume and left ventricular relaxation time constant (tau) following HFD intake. Furthermore, a HFD resulted in increased ERS, and a downregulation of the autophagy and mitophagy level. The present study investigated cardiac function in obese HFD-fed mice. These results aid the pursuit of novel therapeutic targets to combat obesity-associated cardiomyopathy. D.A. Spandidos 2018-09 2018-07-23 /pmc/articles/PMC6102660/ /pubmed/30066870 http://dx.doi.org/10.3892/mmr.2018.9301 Text en Copyright: © Che et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Che, Yan Wang, Zhao-Peng Yuan, Yuan Zhang, Ning Jin, Ya-Ge Wan, Chun-Xia Tang, Qi-Zhu Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title | Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title_full | Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title_fullStr | Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title_full_unstemmed | Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title_short | Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction |
title_sort | role of autophagy in a model of obesity: a long-term high fat diet induces cardiac dysfunction |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102660/ https://www.ncbi.nlm.nih.gov/pubmed/30066870 http://dx.doi.org/10.3892/mmr.2018.9301 |
work_keys_str_mv | AT cheyan roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT wangzhaopeng roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT yuanyuan roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT zhangning roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT jinyage roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT wanchunxia roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction AT tangqizhu roleofautophagyinamodelofobesityalongtermhighfatdietinducescardiacdysfunction |