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Investigation of the role of cullin 4A overexpression in human liver cancer

Cullin 4A (CUL4A) is the major component of cullin-RING-based E3 ubiquitin-protein ligase complexes, which regulate the ubiquitination of target proteins. The overexpression of CUL4A has been associated with the development and progression of various cancer types. However, a detailed understanding o...

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Autores principales: Chen, Gang, Zhao, Xiongqi, Tan, Zedan, Wang, Dongdong, Luo, Ding, Zhang, Peiyao, Cao, Jun, Wang, Fan, Liu, Qiyu, Li, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102737/
https://www.ncbi.nlm.nih.gov/pubmed/30015884
http://dx.doi.org/10.3892/mmr.2018.9233
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author Chen, Gang
Zhao, Xiongqi
Tan, Zedan
Wang, Dongdong
Luo, Ding
Zhang, Peiyao
Cao, Jun
Wang, Fan
Liu, Qiyu
Li, Li
author_facet Chen, Gang
Zhao, Xiongqi
Tan, Zedan
Wang, Dongdong
Luo, Ding
Zhang, Peiyao
Cao, Jun
Wang, Fan
Liu, Qiyu
Li, Li
author_sort Chen, Gang
collection PubMed
description Cullin 4A (CUL4A) is the major component of cullin-RING-based E3 ubiquitin-protein ligase complexes, which regulate the ubiquitination of target proteins. The overexpression of CUL4A has been associated with the development and progression of various cancer types. However, a detailed understanding of the role of CUL4A in human liver cancer has not been determined by previous studies. In the present study, the association between human liver cancer and CUL4A expression was investigated. The expression of CUL4A in liver cancer tissues and paracancerous tissues of patients was investigated by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Overexpression and knockdown of CUL4A were induced with an overexpression vector and small interfering RNA transfection, respectively, in human liver cancer cell lines, and the effects on cell proliferation were analyzed by a Cell Counting Kit-8 assay to investigate the role of CUL4A in human liver cancer. Cell migration, invasion, apoptosis and the cell cycle were also analyzed following transfection. The results of the present study revealed that the mRNA and protein expression of CUL4A was increased in the liver cancer tissues compared with the paracancerous tissues of 3 patients. Additionally, the results demonstrated that downregulation of CUL4A expression inhibited cell proliferation, migration and invasion, and increased the percentage of cell apoptosis, in HEPG2 and MHCC97-H cells, while CUL4A overexpression led to the opposite effects. Therefore, the results of the current study indicated that CUL4A may serve an important role in the development and progression of human liver cancer, and highlights the potential of CUL4A as a novel target in the diagnosis and treatment of human liver cancer and potentially other cancer types.
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spelling pubmed-61027372018-08-23 Investigation of the role of cullin 4A overexpression in human liver cancer Chen, Gang Zhao, Xiongqi Tan, Zedan Wang, Dongdong Luo, Ding Zhang, Peiyao Cao, Jun Wang, Fan Liu, Qiyu Li, Li Mol Med Rep Articles Cullin 4A (CUL4A) is the major component of cullin-RING-based E3 ubiquitin-protein ligase complexes, which regulate the ubiquitination of target proteins. The overexpression of CUL4A has been associated with the development and progression of various cancer types. However, a detailed understanding of the role of CUL4A in human liver cancer has not been determined by previous studies. In the present study, the association between human liver cancer and CUL4A expression was investigated. The expression of CUL4A in liver cancer tissues and paracancerous tissues of patients was investigated by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Overexpression and knockdown of CUL4A were induced with an overexpression vector and small interfering RNA transfection, respectively, in human liver cancer cell lines, and the effects on cell proliferation were analyzed by a Cell Counting Kit-8 assay to investigate the role of CUL4A in human liver cancer. Cell migration, invasion, apoptosis and the cell cycle were also analyzed following transfection. The results of the present study revealed that the mRNA and protein expression of CUL4A was increased in the liver cancer tissues compared with the paracancerous tissues of 3 patients. Additionally, the results demonstrated that downregulation of CUL4A expression inhibited cell proliferation, migration and invasion, and increased the percentage of cell apoptosis, in HEPG2 and MHCC97-H cells, while CUL4A overexpression led to the opposite effects. Therefore, the results of the current study indicated that CUL4A may serve an important role in the development and progression of human liver cancer, and highlights the potential of CUL4A as a novel target in the diagnosis and treatment of human liver cancer and potentially other cancer types. D.A. Spandidos 2018-09 2018-06-29 /pmc/articles/PMC6102737/ /pubmed/30015884 http://dx.doi.org/10.3892/mmr.2018.9233 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Gang
Zhao, Xiongqi
Tan, Zedan
Wang, Dongdong
Luo, Ding
Zhang, Peiyao
Cao, Jun
Wang, Fan
Liu, Qiyu
Li, Li
Investigation of the role of cullin 4A overexpression in human liver cancer
title Investigation of the role of cullin 4A overexpression in human liver cancer
title_full Investigation of the role of cullin 4A overexpression in human liver cancer
title_fullStr Investigation of the role of cullin 4A overexpression in human liver cancer
title_full_unstemmed Investigation of the role of cullin 4A overexpression in human liver cancer
title_short Investigation of the role of cullin 4A overexpression in human liver cancer
title_sort investigation of the role of cullin 4a overexpression in human liver cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102737/
https://www.ncbi.nlm.nih.gov/pubmed/30015884
http://dx.doi.org/10.3892/mmr.2018.9233
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