Cargando…
Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells
BACKGROUND: Bone tissue is one of the main sites for breast metastasis; patients diagnosed with advanced breast cancer mostly develop bone metastasis characterized by severe osteolytic lesions, which heavily influence their life quality. Low Intensity Pulsed Ultrasound (LIPUS) is a form of mechanica...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102871/ https://www.ncbi.nlm.nih.gov/pubmed/30126457 http://dx.doi.org/10.1186/s13046-018-0868-2 |
_version_ | 1783349257177137152 |
---|---|
author | Carina, Valeria Costa, Viviana Pagani, Stefania De Luca, Angela Raimondi, Lavinia Bellavia, Daniele Setti, Stefania Fini, Milena Giavaresi, Gianluca |
author_facet | Carina, Valeria Costa, Viviana Pagani, Stefania De Luca, Angela Raimondi, Lavinia Bellavia, Daniele Setti, Stefania Fini, Milena Giavaresi, Gianluca |
author_sort | Carina, Valeria |
collection | PubMed |
description | BACKGROUND: Bone tissue is one of the main sites for breast metastasis; patients diagnosed with advanced breast cancer mostly develop bone metastasis characterized by severe osteolytic lesions, which heavily influence their life quality. Low Intensity Pulsed Ultrasound (LIPUS) is a form of mechanical energy able to modulate various molecular pathways both in cancer and in health cells. The purpose of the present study was to evaluate for the first time, the ability of LIPUS to modulate osteolytic capability of breast cancer cells. METHODS: Two different approaches were employed: a) Indirect method -conditioned medium obtained by MDA-MB-231 cell line treated or untreated with LIPUS was used to induce osteoclast differentiation of murine macrophage Raw264.7 cell line; and b) Direct method -MDA-MB-231 were co-cultured with Raw264.7 cells and treated or untreated with LIPUS. RESULTS: LIPUS treatment impaired MDA-MB-231 cell dependentosteoclast differentiation and produced a reduction of osteoclast markers such as Cathepsin K, Matrix Metalloproteinase 9 and Tartrate Resistant Acid Phosphatase, suggesting its role as an effective and safe adjuvant in bone metastasis management. CONCLUSION: LIPUS treatment could be a good and safety therapeutic adjuvant in osteolyitic bone metastasis not only for the induction properties of bone regeneration, but also for the reduction of osteolysis. |
format | Online Article Text |
id | pubmed-6102871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61028712018-08-27 Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells Carina, Valeria Costa, Viviana Pagani, Stefania De Luca, Angela Raimondi, Lavinia Bellavia, Daniele Setti, Stefania Fini, Milena Giavaresi, Gianluca J Exp Clin Cancer Res Research BACKGROUND: Bone tissue is one of the main sites for breast metastasis; patients diagnosed with advanced breast cancer mostly develop bone metastasis characterized by severe osteolytic lesions, which heavily influence their life quality. Low Intensity Pulsed Ultrasound (LIPUS) is a form of mechanical energy able to modulate various molecular pathways both in cancer and in health cells. The purpose of the present study was to evaluate for the first time, the ability of LIPUS to modulate osteolytic capability of breast cancer cells. METHODS: Two different approaches were employed: a) Indirect method -conditioned medium obtained by MDA-MB-231 cell line treated or untreated with LIPUS was used to induce osteoclast differentiation of murine macrophage Raw264.7 cell line; and b) Direct method -MDA-MB-231 were co-cultured with Raw264.7 cells and treated or untreated with LIPUS. RESULTS: LIPUS treatment impaired MDA-MB-231 cell dependentosteoclast differentiation and produced a reduction of osteoclast markers such as Cathepsin K, Matrix Metalloproteinase 9 and Tartrate Resistant Acid Phosphatase, suggesting its role as an effective and safe adjuvant in bone metastasis management. CONCLUSION: LIPUS treatment could be a good and safety therapeutic adjuvant in osteolyitic bone metastasis not only for the induction properties of bone regeneration, but also for the reduction of osteolysis. BioMed Central 2018-08-20 /pmc/articles/PMC6102871/ /pubmed/30126457 http://dx.doi.org/10.1186/s13046-018-0868-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Carina, Valeria Costa, Viviana Pagani, Stefania De Luca, Angela Raimondi, Lavinia Bellavia, Daniele Setti, Stefania Fini, Milena Giavaresi, Gianluca Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title | Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title_full | Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title_fullStr | Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title_full_unstemmed | Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title_short | Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
title_sort | inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102871/ https://www.ncbi.nlm.nih.gov/pubmed/30126457 http://dx.doi.org/10.1186/s13046-018-0868-2 |
work_keys_str_mv | AT carinavaleria inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT costaviviana inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT paganistefania inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT delucaangela inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT raimondilavinia inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT bellaviadaniele inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT settistefania inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT finimilena inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells AT giavaresigianluca inhibitoryeffectsoflowintensitypulsedultrasoundonosteoclastogenesisinducedinvitrobybreastcancercells |