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Fine details in complex environments: the power of cryo-electron tomography
Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6103461/ https://www.ncbi.nlm.nih.gov/pubmed/29934301 http://dx.doi.org/10.1042/BST20170351 |
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author | Hutchings, Joshua Zanetti, Giulia |
author_facet | Hutchings, Joshua Zanetti, Giulia |
author_sort | Hutchings, Joshua |
collection | PubMed |
description | Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experiment, a varying degree of resolutions can be achieved, with the first near-atomic molecular structures becoming recently available. The power of CET has increased significantly in the last 5 years, in parallel with improvements in cryo-EM hardware and software that have also benefited single-particle reconstruction techniques. In this review, we cover the typical CET pipeline, starting from sample preparation, to data collection and processing, and highlight in particular the recent developments that support structural biology in situ. We provide some examples that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. |
format | Online Article Text |
id | pubmed-6103461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61034612018-09-05 Fine details in complex environments: the power of cryo-electron tomography Hutchings, Joshua Zanetti, Giulia Biochem Soc Trans Review Articles Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experiment, a varying degree of resolutions can be achieved, with the first near-atomic molecular structures becoming recently available. The power of CET has increased significantly in the last 5 years, in parallel with improvements in cryo-EM hardware and software that have also benefited single-particle reconstruction techniques. In this review, we cover the typical CET pipeline, starting from sample preparation, to data collection and processing, and highlight in particular the recent developments that support structural biology in situ. We provide some examples that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. Portland Press Ltd. 2018-08-20 2018-06-22 /pmc/articles/PMC6103461/ /pubmed/29934301 http://dx.doi.org/10.1042/BST20170351 Text en © 2018 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Articles Hutchings, Joshua Zanetti, Giulia Fine details in complex environments: the power of cryo-electron tomography |
title | Fine details in complex environments: the power of cryo-electron tomography |
title_full | Fine details in complex environments: the power of cryo-electron tomography |
title_fullStr | Fine details in complex environments: the power of cryo-electron tomography |
title_full_unstemmed | Fine details in complex environments: the power of cryo-electron tomography |
title_short | Fine details in complex environments: the power of cryo-electron tomography |
title_sort | fine details in complex environments: the power of cryo-electron tomography |
topic | Review Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6103461/ https://www.ncbi.nlm.nih.gov/pubmed/29934301 http://dx.doi.org/10.1042/BST20170351 |
work_keys_str_mv | AT hutchingsjoshua finedetailsincomplexenvironmentsthepowerofcryoelectrontomography AT zanettigiulia finedetailsincomplexenvironmentsthepowerofcryoelectrontomography |