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HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis

BACKGROUND: The expression of high temperature requirement factor A1 (Htra1) has been reported to be decreased in ovarian carcinoma, but its prognostic effect remains undetermined. METHODS: We evaluated the impact of HtrA1 downregulation in tumoral tissues on cancer progression and death in women wi...

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Autores principales: Gagné, Andréanne, Têtu, Bernard, Orain, Michèle, Turcotte, Stéphane, Plante, Marie, Grégoire, Jean, Renaud, Marie-Claude, Bairati, Isabelle, Trueel, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104006/
https://www.ncbi.nlm.nih.gov/pubmed/30131069
http://dx.doi.org/10.1186/s13000-018-0736-6
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author Gagné, Andréanne
Têtu, Bernard
Orain, Michèle
Turcotte, Stéphane
Plante, Marie
Grégoire, Jean
Renaud, Marie-Claude
Bairati, Isabelle
Trueel, Dominique
author_facet Gagné, Andréanne
Têtu, Bernard
Orain, Michèle
Turcotte, Stéphane
Plante, Marie
Grégoire, Jean
Renaud, Marie-Claude
Bairati, Isabelle
Trueel, Dominique
author_sort Gagné, Andréanne
collection PubMed
description BACKGROUND: The expression of high temperature requirement factor A1 (Htra1) has been reported to be decreased in ovarian carcinoma, but its prognostic effect remains undetermined. METHODS: We evaluated the impact of HtrA1 downregulation in tumoral tissues on cancer progression and death in women with serous ovarian carcinoma. HtrA1 staining was performed on tissue microarrays (TMA) comprised of tumor samples from a cohort of 106 women who were diagnosed with primary high-grade serous ovarian carcinoma and receiving standard treatment at the Québec University Hospital between 1993 and 2006. HtrA1 expression was assessed visually (percentage of positive nuclei) and by digital image analysis (percentage of positive area). Cox regression multivariate models included standard prognostic factors and were used to estimate adjusted hazard ratios (aHR) for progression or death in the cohort. RESULTS: By visual analysis, a low percentage of HtrA1-positive nuclei (< 10% vs ≥10%) tend to be associated with a lower risk of progression (aHR = 0.71; 95% Confidence interval (CI) = 0.46–1.09; P = 0.11) and mortality (aHR = 0.65; 95% CI = 0.41–1.04; P = 0.07). Low nuclear HtrA1 expression assessed by digital image analysis (< median % vs ≥ median %) showed a significant association with lower risk of progression (aHR = 0.62; 95% CI = 0.40–0.95; p = 0.03) and death (aHR = 0.60; 95% CI = 0.38–0.95; p = 0.03). CONCLUSION: Altogether, our results demonstrate that nuclear downregulation of HtrA1 is associated with a better prognosis in women with high grade serous ovarian carcinoma.
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spelling pubmed-61040062018-08-30 HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis Gagné, Andréanne Têtu, Bernard Orain, Michèle Turcotte, Stéphane Plante, Marie Grégoire, Jean Renaud, Marie-Claude Bairati, Isabelle Trueel, Dominique Diagn Pathol Research BACKGROUND: The expression of high temperature requirement factor A1 (Htra1) has been reported to be decreased in ovarian carcinoma, but its prognostic effect remains undetermined. METHODS: We evaluated the impact of HtrA1 downregulation in tumoral tissues on cancer progression and death in women with serous ovarian carcinoma. HtrA1 staining was performed on tissue microarrays (TMA) comprised of tumor samples from a cohort of 106 women who were diagnosed with primary high-grade serous ovarian carcinoma and receiving standard treatment at the Québec University Hospital between 1993 and 2006. HtrA1 expression was assessed visually (percentage of positive nuclei) and by digital image analysis (percentage of positive area). Cox regression multivariate models included standard prognostic factors and were used to estimate adjusted hazard ratios (aHR) for progression or death in the cohort. RESULTS: By visual analysis, a low percentage of HtrA1-positive nuclei (< 10% vs ≥10%) tend to be associated with a lower risk of progression (aHR = 0.71; 95% Confidence interval (CI) = 0.46–1.09; P = 0.11) and mortality (aHR = 0.65; 95% CI = 0.41–1.04; P = 0.07). Low nuclear HtrA1 expression assessed by digital image analysis (< median % vs ≥ median %) showed a significant association with lower risk of progression (aHR = 0.62; 95% CI = 0.40–0.95; p = 0.03) and death (aHR = 0.60; 95% CI = 0.38–0.95; p = 0.03). CONCLUSION: Altogether, our results demonstrate that nuclear downregulation of HtrA1 is associated with a better prognosis in women with high grade serous ovarian carcinoma. BioMed Central 2018-08-21 /pmc/articles/PMC6104006/ /pubmed/30131069 http://dx.doi.org/10.1186/s13000-018-0736-6 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Gagné, Andréanne
Têtu, Bernard
Orain, Michèle
Turcotte, Stéphane
Plante, Marie
Grégoire, Jean
Renaud, Marie-Claude
Bairati, Isabelle
Trueel, Dominique
HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title_full HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title_fullStr HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title_full_unstemmed HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title_short HtrA1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
title_sort htra1 expression and the prognosis of high-grade serous ovarian carcinoma: a cohort study using digital analysis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104006/
https://www.ncbi.nlm.nih.gov/pubmed/30131069
http://dx.doi.org/10.1186/s13000-018-0736-6
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