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mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii

Regulation of gene expression at the translational level allows rapid adaptation of cellular proteomes to quickly changing environmental conditions and is thus central for prokaryotic organisms. Small non-coding RNAs (sRNAs) have been reported to effectively orchestrate translation control in bacter...

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Autores principales: Wyss, Leander, Waser, Melanie, Gebetsberger, Jennifer, Zywicki, Marek, Polacek, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104027/
https://www.ncbi.nlm.nih.gov/pubmed/30131517
http://dx.doi.org/10.1038/s41598-018-30332-w
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author Wyss, Leander
Waser, Melanie
Gebetsberger, Jennifer
Zywicki, Marek
Polacek, Norbert
author_facet Wyss, Leander
Waser, Melanie
Gebetsberger, Jennifer
Zywicki, Marek
Polacek, Norbert
author_sort Wyss, Leander
collection PubMed
description Regulation of gene expression at the translational level allows rapid adaptation of cellular proteomes to quickly changing environmental conditions and is thus central for prokaryotic organisms. Small non-coding RNAs (sRNAs) have been reported to effectively orchestrate translation control in bacteria and archaea mainly by targeting mRNAs by partial base complementarity. Here we report an unprecedented mechanism how sRNAs are capable of modulating protein biosynthesis in the halophilic archaeon Haloferax volcanii. By analyzing the ribosome-associated ncRNAs (rancRNAs) under different stress conditions we identified an intergenic sRNA, termed rancRNA_s194, that is primarily expressed during exponential growth under all tested conditions. By interaction with the ribosome rancRNA_s194 inhibits peptide bond formation and protein synthesis in vitro but appears to target a specific mRNA in vivo. The respective knock-out strain shows a reduced lag phase in media containing xylose as sole carbon source and outcompetes the wildtype cells under these conditions. Mass spectrometry, polysome profiling and mRNA binding competition experiments suggest that rancRNA_s194 prevents the cstA mRNA from being efficiently translated by H. volcanii ribosomes. These findings enlarge the regulatory repertoire of archaeal sRNAs in modulating post-transcriptional gene expression.
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spelling pubmed-61040272018-08-27 mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii Wyss, Leander Waser, Melanie Gebetsberger, Jennifer Zywicki, Marek Polacek, Norbert Sci Rep Article Regulation of gene expression at the translational level allows rapid adaptation of cellular proteomes to quickly changing environmental conditions and is thus central for prokaryotic organisms. Small non-coding RNAs (sRNAs) have been reported to effectively orchestrate translation control in bacteria and archaea mainly by targeting mRNAs by partial base complementarity. Here we report an unprecedented mechanism how sRNAs are capable of modulating protein biosynthesis in the halophilic archaeon Haloferax volcanii. By analyzing the ribosome-associated ncRNAs (rancRNAs) under different stress conditions we identified an intergenic sRNA, termed rancRNA_s194, that is primarily expressed during exponential growth under all tested conditions. By interaction with the ribosome rancRNA_s194 inhibits peptide bond formation and protein synthesis in vitro but appears to target a specific mRNA in vivo. The respective knock-out strain shows a reduced lag phase in media containing xylose as sole carbon source and outcompetes the wildtype cells under these conditions. Mass spectrometry, polysome profiling and mRNA binding competition experiments suggest that rancRNA_s194 prevents the cstA mRNA from being efficiently translated by H. volcanii ribosomes. These findings enlarge the regulatory repertoire of archaeal sRNAs in modulating post-transcriptional gene expression. Nature Publishing Group UK 2018-08-21 /pmc/articles/PMC6104027/ /pubmed/30131517 http://dx.doi.org/10.1038/s41598-018-30332-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wyss, Leander
Waser, Melanie
Gebetsberger, Jennifer
Zywicki, Marek
Polacek, Norbert
mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title_full mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title_fullStr mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title_full_unstemmed mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title_short mRNA-specific translation regulation by a ribosome-associated ncRNA in Haloferax volcanii
title_sort mrna-specific translation regulation by a ribosome-associated ncrna in haloferax volcanii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104027/
https://www.ncbi.nlm.nih.gov/pubmed/30131517
http://dx.doi.org/10.1038/s41598-018-30332-w
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