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Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis

Nitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and glycine betaine content in response to inhibition of nitric oxide synthase-like act...

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Autores principales: Phillips, Kyle, Majola, Anelisa, Gokul, Arun, Keyster, Marshall, Ludidi, Ndiko, Egbichi, Ifeanyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105647/
https://www.ncbi.nlm.nih.gov/pubmed/30135488
http://dx.doi.org/10.1038/s41598-018-31131-z
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author Phillips, Kyle
Majola, Anelisa
Gokul, Arun
Keyster, Marshall
Ludidi, Ndiko
Egbichi, Ifeanyi
author_facet Phillips, Kyle
Majola, Anelisa
Gokul, Arun
Keyster, Marshall
Ludidi, Ndiko
Egbichi, Ifeanyi
author_sort Phillips, Kyle
collection PubMed
description Nitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and glycine betaine content in response to inhibition of nitric oxide synthase-like activity are associated with transcriptional regulation by analyzing transcript levels of genes (betaine aldehyde dehydrogenase) involved in glycine betaine biosynthesis and those encoding antioxidant enzymes (ascorbate peroxidase and catalase) in leaves of maize seedlings treated with an inhibitor of nitric oxide synthase-like activity. In seedlings treated with a nitric oxide synthase inhibitor, transcript levels of betaine aldehyde dehydrogenase were decreased. In plants treated with the nitric oxide synthase inhibitor, the transcript levels of ascorbate peroxidase-encoding genes were down-regulated. We thus conclude that inhibition of nitric oxide synthase-like activity suppresses the expression of ascorbate peroxidase and betaine aldehyde dehydrogenase genes in maize leaves. Furthermore, catalase activity was suppressed in leaves of plants treated with nitric oxide synthase inhibitor; and this corresponded with the suppression of the expression of catalase genes. We further conclude that inhibition of nitric oxide synthase-like activity, which suppresses ascorbate peroxidase and catalase enzymatic activities, results in increased H(2)O(2) content.
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spelling pubmed-61056472018-08-27 Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis Phillips, Kyle Majola, Anelisa Gokul, Arun Keyster, Marshall Ludidi, Ndiko Egbichi, Ifeanyi Sci Rep Article Nitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and glycine betaine content in response to inhibition of nitric oxide synthase-like activity are associated with transcriptional regulation by analyzing transcript levels of genes (betaine aldehyde dehydrogenase) involved in glycine betaine biosynthesis and those encoding antioxidant enzymes (ascorbate peroxidase and catalase) in leaves of maize seedlings treated with an inhibitor of nitric oxide synthase-like activity. In seedlings treated with a nitric oxide synthase inhibitor, transcript levels of betaine aldehyde dehydrogenase were decreased. In plants treated with the nitric oxide synthase inhibitor, the transcript levels of ascorbate peroxidase-encoding genes were down-regulated. We thus conclude that inhibition of nitric oxide synthase-like activity suppresses the expression of ascorbate peroxidase and betaine aldehyde dehydrogenase genes in maize leaves. Furthermore, catalase activity was suppressed in leaves of plants treated with nitric oxide synthase inhibitor; and this corresponded with the suppression of the expression of catalase genes. We further conclude that inhibition of nitric oxide synthase-like activity, which suppresses ascorbate peroxidase and catalase enzymatic activities, results in increased H(2)O(2) content. Nature Publishing Group UK 2018-08-22 /pmc/articles/PMC6105647/ /pubmed/30135488 http://dx.doi.org/10.1038/s41598-018-31131-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Phillips, Kyle
Majola, Anelisa
Gokul, Arun
Keyster, Marshall
Ludidi, Ndiko
Egbichi, Ifeanyi
Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title_full Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title_fullStr Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title_full_unstemmed Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title_short Inhibition of NOS- like activity in maize alters the expression of genes involved in H(2)O(2) scavenging and glycine betaine biosynthesis
title_sort inhibition of nos- like activity in maize alters the expression of genes involved in h(2)o(2) scavenging and glycine betaine biosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105647/
https://www.ncbi.nlm.nih.gov/pubmed/30135488
http://dx.doi.org/10.1038/s41598-018-31131-z
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