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Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development

Treatment of fetal rat and embryonic chicken with exogenous glucocorticoids induces premature differentiation of growth hormone (GH) secreting cells. The effect of corticosterone (CORT) on somatotroph differentiation was mostly studied in pituitary cells in vitro. Currently, there is no evidence for...

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Autores principales: YU, Jianning, YAN, Leyan, CHEN, Zhe, LI, Hui, ZHU, Huanxi, CHEN, Rong, SHI, ZhenDan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105744/
https://www.ncbi.nlm.nih.gov/pubmed/29887536
http://dx.doi.org/10.1262/jrd.2018-001
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author YU, Jianning
YAN, Leyan
CHEN, Zhe
LI, Hui
ZHU, Huanxi
CHEN, Rong
SHI, ZhenDan
author_facet YU, Jianning
YAN, Leyan
CHEN, Zhe
LI, Hui
ZHU, Huanxi
CHEN, Rong
SHI, ZhenDan
author_sort YU, Jianning
collection PubMed
description Treatment of fetal rat and embryonic chicken with exogenous glucocorticoids induces premature differentiation of growth hormone (GH) secreting cells. The effect of corticosterone (CORT) on somatotroph differentiation was mostly studied in pituitary cells in vitro. Currently, there is no evidence for glucocorticoid-mediated induction of somatotroph differentiation during pituitary development in bird species other than chicken. In this study, we sought to find out if in ovo injection of corticosterone into developing goose embryos could induce premature increase of GH in somatotrophs. On embryonic day (e) 15, the albumen of fertile goose eggs was injected with 300 μl of 0.9% saline, 300 μl 5 × 10(–8)M CORT, or 300 μl 5 × 10(–6) M CORT. Embryos were allowed to develop until e20 and e28 and isolated pituitaries were subjected to quantitative real-time PCR and immunocytochemistry to detect GH mRNA and protein, respectively. At e20 and e28, blood from chorioallantoic vessels was subjected to radioimmunoassay for analysis of plasma GH protein. In ovo administration of exogenous corticosterone brought about a 2.5-fold increase in the expression of GH mRNA and increased the in situ expression of GH protein in goose pituitary cells, and enhanced plasma GH levels compared to that of the respective controls at e20. These findings prove that administration of glucocorticoid could stimulate the expression of GH in somatotrophs during goose embryonic development. Our results suggest the probable involvement of membrane glucocorticoid receptor in the corticosterone mediated expression of GH. Together with previously published data, our results suggest that corticosterone mediated induction of GH expression during embryonic development is relatively conserved among different vertebrates.
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spelling pubmed-61057442018-08-27 Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development YU, Jianning YAN, Leyan CHEN, Zhe LI, Hui ZHU, Huanxi CHEN, Rong SHI, ZhenDan J Reprod Dev Original Article Treatment of fetal rat and embryonic chicken with exogenous glucocorticoids induces premature differentiation of growth hormone (GH) secreting cells. The effect of corticosterone (CORT) on somatotroph differentiation was mostly studied in pituitary cells in vitro. Currently, there is no evidence for glucocorticoid-mediated induction of somatotroph differentiation during pituitary development in bird species other than chicken. In this study, we sought to find out if in ovo injection of corticosterone into developing goose embryos could induce premature increase of GH in somatotrophs. On embryonic day (e) 15, the albumen of fertile goose eggs was injected with 300 μl of 0.9% saline, 300 μl 5 × 10(–8)M CORT, or 300 μl 5 × 10(–6) M CORT. Embryos were allowed to develop until e20 and e28 and isolated pituitaries were subjected to quantitative real-time PCR and immunocytochemistry to detect GH mRNA and protein, respectively. At e20 and e28, blood from chorioallantoic vessels was subjected to radioimmunoassay for analysis of plasma GH protein. In ovo administration of exogenous corticosterone brought about a 2.5-fold increase in the expression of GH mRNA and increased the in situ expression of GH protein in goose pituitary cells, and enhanced plasma GH levels compared to that of the respective controls at e20. These findings prove that administration of glucocorticoid could stimulate the expression of GH in somatotrophs during goose embryonic development. Our results suggest the probable involvement of membrane glucocorticoid receptor in the corticosterone mediated expression of GH. Together with previously published data, our results suggest that corticosterone mediated induction of GH expression during embryonic development is relatively conserved among different vertebrates. The Society for Reproduction and Development 2018-06-08 2018-08 /pmc/articles/PMC6105744/ /pubmed/29887536 http://dx.doi.org/10.1262/jrd.2018-001 Text en ©2018 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
YU, Jianning
YAN, Leyan
CHEN, Zhe
LI, Hui
ZHU, Huanxi
CHEN, Rong
SHI, ZhenDan
Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title_full Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title_fullStr Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title_full_unstemmed Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title_short Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
title_sort corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105744/
https://www.ncbi.nlm.nih.gov/pubmed/29887536
http://dx.doi.org/10.1262/jrd.2018-001
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