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A cautionary note for researchers treating mice with the neurotransmitter norepinephrine

The sympathetic nervous system plays a crucial role in metabolic function and glucose homeostasis. Norepinephrine is the main neurotransmitter released from sympathetic neurons. The major goal of our studies was to examine the impact of norepinephrine on metabolism related gene expression in obesity...

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Autores principales: Matthews, Vance B., Rudnicka, Caroline, Schlaich, Markus P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105757/
https://www.ncbi.nlm.nih.gov/pubmed/30148215
http://dx.doi.org/10.1016/j.bbrep.2018.08.003
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author Matthews, Vance B.
Rudnicka, Caroline
Schlaich, Markus P.
author_facet Matthews, Vance B.
Rudnicka, Caroline
Schlaich, Markus P.
author_sort Matthews, Vance B.
collection PubMed
description The sympathetic nervous system plays a crucial role in metabolic function and glucose homeostasis. Norepinephrine is the main neurotransmitter released from sympathetic neurons. The major goal of our studies was to examine the impact of norepinephrine on metabolism related gene expression in obesity in vivo. Interestingly, we discovered that norepinephrine had a detrimental effect in our studies. C57BL6/J mice fed a high fat diet were intraperitoneally injected with 0.2 or 2 mg/kg/day norepinephrine. These doses of norepinephrine have been used previously by other researchers. Survival of the mice was documented. Kidney and bladder tissues were excised and fixed for histological studies. A subset of norepinephrine treated mice experienced unexpected adverse events which included bladder distension and reduced kidney perfusion as suggested by kidney discolouration. This eventuated in the mice having to be sacrificed or the mice succumbed to the pathological condition. To our knowledge, such an effect of norepinephrine has not been previously reported in mice. Morphological examination of kidney and bladder indicated marked detrimental architectural changes, which we postulate is associated with norepinephrine induced vasoconstriction, urinary retention and renal impairment. Our studies highlight that administration of norepinephrine to mice may trigger adverse effects relating predominantly to the urogenital tract which can result in decline in a subpopulation of these mice. Researchers administering norepinephrine in mouse models should be aware and look out for these unexpected adverse events associated with the use of norepinephrine.
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spelling pubmed-61057572018-08-24 A cautionary note for researchers treating mice with the neurotransmitter norepinephrine Matthews, Vance B. Rudnicka, Caroline Schlaich, Markus P. Biochem Biophys Rep Research Article The sympathetic nervous system plays a crucial role in metabolic function and glucose homeostasis. Norepinephrine is the main neurotransmitter released from sympathetic neurons. The major goal of our studies was to examine the impact of norepinephrine on metabolism related gene expression in obesity in vivo. Interestingly, we discovered that norepinephrine had a detrimental effect in our studies. C57BL6/J mice fed a high fat diet were intraperitoneally injected with 0.2 or 2 mg/kg/day norepinephrine. These doses of norepinephrine have been used previously by other researchers. Survival of the mice was documented. Kidney and bladder tissues were excised and fixed for histological studies. A subset of norepinephrine treated mice experienced unexpected adverse events which included bladder distension and reduced kidney perfusion as suggested by kidney discolouration. This eventuated in the mice having to be sacrificed or the mice succumbed to the pathological condition. To our knowledge, such an effect of norepinephrine has not been previously reported in mice. Morphological examination of kidney and bladder indicated marked detrimental architectural changes, which we postulate is associated with norepinephrine induced vasoconstriction, urinary retention and renal impairment. Our studies highlight that administration of norepinephrine to mice may trigger adverse effects relating predominantly to the urogenital tract which can result in decline in a subpopulation of these mice. Researchers administering norepinephrine in mouse models should be aware and look out for these unexpected adverse events associated with the use of norepinephrine. Elsevier 2018-08-17 /pmc/articles/PMC6105757/ /pubmed/30148215 http://dx.doi.org/10.1016/j.bbrep.2018.08.003 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Matthews, Vance B.
Rudnicka, Caroline
Schlaich, Markus P.
A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title_full A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title_fullStr A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title_full_unstemmed A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title_short A cautionary note for researchers treating mice with the neurotransmitter norepinephrine
title_sort cautionary note for researchers treating mice with the neurotransmitter norepinephrine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105757/
https://www.ncbi.nlm.nih.gov/pubmed/30148215
http://dx.doi.org/10.1016/j.bbrep.2018.08.003
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