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Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence
Ribonucleic acid (RNA)-silencing mechanisms exist in many eukaryotes to regulate a variety of biological processes. The known molecular components are related to Dicers, Argonautes and RNA-dependent RNA polymerases. Previous biochemical studies have also suggested that Qip, with an exonuclease domai...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6106068/ https://www.ncbi.nlm.nih.gov/pubmed/30151321 http://dx.doi.org/10.1080/21501203.2015.1027313 |
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author | Cheng, Lin Ling, Jian Liang, Liqin Luo, Zhongqin Zhang, Jie Xie, Bingyan |
author_facet | Cheng, Lin Ling, Jian Liang, Liqin Luo, Zhongqin Zhang, Jie Xie, Bingyan |
author_sort | Cheng, Lin |
collection | PubMed |
description | Ribonucleic acid (RNA)-silencing mechanisms exist in many eukaryotes to regulate a variety of biological processes. The known molecular components are related to Dicers, Argonautes and RNA-dependent RNA polymerases. Previous biochemical studies have also suggested that Qip, with an exonuclease domain, facilitates the conversion of duplex small interfering RNAs into single strands. In our study, the Qip gene in Fusarium oxysporum was disrupted using homologous recombination technology. The deletion of the Qip gene resulted in a decrease in colony growth rates but increased the number of branches. Additionally, the ΔQip mutant had a reduced pathogenicity in cabbage. Our results show Qip gene in F. oxysporum is required for normal hyphae morphology and virulence. The mutant will be useful for elucidating the relationship between the RNA-silencing mechanism and hyphal growth and development in F. oxysporum. |
format | Online Article Text |
id | pubmed-6106068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61060682018-08-27 Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence Cheng, Lin Ling, Jian Liang, Liqin Luo, Zhongqin Zhang, Jie Xie, Bingyan Mycology Original Article Ribonucleic acid (RNA)-silencing mechanisms exist in many eukaryotes to regulate a variety of biological processes. The known molecular components are related to Dicers, Argonautes and RNA-dependent RNA polymerases. Previous biochemical studies have also suggested that Qip, with an exonuclease domain, facilitates the conversion of duplex small interfering RNAs into single strands. In our study, the Qip gene in Fusarium oxysporum was disrupted using homologous recombination technology. The deletion of the Qip gene resulted in a decrease in colony growth rates but increased the number of branches. Additionally, the ΔQip mutant had a reduced pathogenicity in cabbage. Our results show Qip gene in F. oxysporum is required for normal hyphae morphology and virulence. The mutant will be useful for elucidating the relationship between the RNA-silencing mechanism and hyphal growth and development in F. oxysporum. Taylor & Francis 2015-03-25 /pmc/articles/PMC6106068/ /pubmed/30151321 http://dx.doi.org/10.1080/21501203.2015.1027313 Text en © 2015 Mycological Society of China http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cheng, Lin Ling, Jian Liang, Liqin Luo, Zhongqin Zhang, Jie Xie, Bingyan Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title | Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title_full | Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title_fullStr | Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title_full_unstemmed | Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title_short | Qip gene in Fusarium oxysporum is required for normal hyphae morphology and virulence |
title_sort | qip gene in fusarium oxysporum is required for normal hyphae morphology and virulence |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6106068/ https://www.ncbi.nlm.nih.gov/pubmed/30151321 http://dx.doi.org/10.1080/21501203.2015.1027313 |
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