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Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping
Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genot...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107157/ https://www.ncbi.nlm.nih.gov/pubmed/30138365 http://dx.doi.org/10.1371/journal.pone.0201981 |
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author | Perea Razo, Claudia Angélica Rodríguez Hernández, Elba Ponce, Sergio Iván Román Milián Suazo, Feliciano Robbe-Austerman, Suelee Stuber, Tod Cantó Alarcón, Germinal Jorge |
author_facet | Perea Razo, Claudia Angélica Rodríguez Hernández, Elba Ponce, Sergio Iván Román Milián Suazo, Feliciano Robbe-Austerman, Suelee Stuber, Tod Cantó Alarcón, Germinal Jorge |
author_sort | Perea Razo, Claudia Angélica |
collection | PubMed |
description | Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce. |
format | Online Article Text |
id | pubmed-6107157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61071572018-08-30 Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping Perea Razo, Claudia Angélica Rodríguez Hernández, Elba Ponce, Sergio Iván Román Milián Suazo, Feliciano Robbe-Austerman, Suelee Stuber, Tod Cantó Alarcón, Germinal Jorge PLoS One Research Article Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce. Public Library of Science 2018-08-23 /pmc/articles/PMC6107157/ /pubmed/30138365 http://dx.doi.org/10.1371/journal.pone.0201981 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Perea Razo, Claudia Angélica Rodríguez Hernández, Elba Ponce, Sergio Iván Román Milián Suazo, Feliciano Robbe-Austerman, Suelee Stuber, Tod Cantó Alarcón, Germinal Jorge Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title_full | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title_fullStr | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title_full_unstemmed | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title_short | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping |
title_sort | molecular epidemiology of cattle tuberculosis in mexico through whole-genome sequencing and spoligotyping |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107157/ https://www.ncbi.nlm.nih.gov/pubmed/30138365 http://dx.doi.org/10.1371/journal.pone.0201981 |
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