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Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays

Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and bio...

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Autores principales: Foncy, Julie, Estève, Aurore, Degache, Amélie, Colin, Camille, Dollat, Xavier, Cau, Jean-Christophe, Vieu, Christophe, Trévisiol, Emmanuelle, Malaquin, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107178/
https://www.ncbi.nlm.nih.gov/pubmed/30138342
http://dx.doi.org/10.1371/journal.pone.0202531
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author Foncy, Julie
Estève, Aurore
Degache, Amélie
Colin, Camille
Dollat, Xavier
Cau, Jean-Christophe
Vieu, Christophe
Trévisiol, Emmanuelle
Malaquin, Laurent
author_facet Foncy, Julie
Estève, Aurore
Degache, Amélie
Colin, Camille
Dollat, Xavier
Cau, Jean-Christophe
Vieu, Christophe
Trévisiol, Emmanuelle
Malaquin, Laurent
author_sort Foncy, Julie
collection PubMed
description Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extracellular matrix proteins for the fabrication of cell microarrays. We have developed a microfluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm(2) through the control of both the flow rate and the wetting mechanism of the stamp surface during microfluidic inking. Thanks to the reproducibility and integration capabilities provided by microfluidics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays.
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spelling pubmed-61071782018-08-30 Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays Foncy, Julie Estève, Aurore Degache, Amélie Colin, Camille Dollat, Xavier Cau, Jean-Christophe Vieu, Christophe Trévisiol, Emmanuelle Malaquin, Laurent PLoS One Research Article Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extracellular matrix proteins for the fabrication of cell microarrays. We have developed a microfluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm(2) through the control of both the flow rate and the wetting mechanism of the stamp surface during microfluidic inking. Thanks to the reproducibility and integration capabilities provided by microfluidics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays. Public Library of Science 2018-08-23 /pmc/articles/PMC6107178/ /pubmed/30138342 http://dx.doi.org/10.1371/journal.pone.0202531 Text en © 2018 Foncy et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Foncy, Julie
Estève, Aurore
Degache, Amélie
Colin, Camille
Dollat, Xavier
Cau, Jean-Christophe
Vieu, Christophe
Trévisiol, Emmanuelle
Malaquin, Laurent
Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title_full Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title_fullStr Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title_full_unstemmed Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title_short Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
title_sort dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107178/
https://www.ncbi.nlm.nih.gov/pubmed/30138342
http://dx.doi.org/10.1371/journal.pone.0202531
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