Cargando…

Optimal reference genes for normalization of qPCR gene expression data from proton and photon irradiated dermal fibroblasts

The transcriptional response of cells exposed to proton radiation is not equivalent to the response induced by traditional photon beams. Changes in cellular signalling is most commonly studied using the method Quantitative polymerase chain reaction (qPCR). Stable reference genes must be used to accu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nielsen, Steffen, Bassler, Niels, Grzanka, Leszek, Swakon, Jan, Olko, Pawel, Andreassen, Christian Nicolaj, Alsner, Jan, Sørensen, Brita Singers
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107545/ https://www.ncbi.nlm.nih.gov/pubmed/30139945 http://dx.doi.org/10.1038/s41598-018-30946-0

Ejemplares similares

Relative Biological Effectiveness of Antiprotons the AD-4/ACE Experiment
por: Holzscheiter, Michael, et al.
Publicado: (2017)

Simultaneous Hypoxia and Low Extracellular pH Suppress Overall Metabolic Rate and Protein Synthesis In Vitro
por: Sørensen, Brita Singers, et al.
Publicado: (2015)

A prognostic profile of hypoxia-induced genes for localised high-grade soft tissue sarcoma
por: Aggerholm-Pedersen, Ninna, et al.
Publicado: (2016)

Plasma proteins as prognostic biomarkers in radiotherapy treated head and neck cancer patients
por: Brøndum, Line, et al.
Publicado: (2017)

Free radicals properties of gamma-irradiated penicillin-derived antibiotics: piperacillin, ampicillin, and crystalline penicillin
por: Wilczyński, Sławomir, et al.
Publicado: (2013)

Proteomic Analysis of Proton Beam Irradiated Human Melanoma Cells
por: Kedracka-Krok, Sylwia, et al.
Publicado: (2014)

Hypomethylation and increased expression of the putative oncogene ELMO3 are associated with lung cancer development and metastases formation
por: Søes, Signe, et al.
Publicado: (2014)

Tumor Hypoxia: Impact on Radiation Therapy and Molecular Pathways
por: Sørensen, Brita Singers, et al.
Publicado: (2020)

The Importance of Reference Gene Analysis of Formalin-Fixed, Paraffin-Embedded Samples from Sarcoma Patients — An Often Underestimated Problem()()
por: Aggerholm-Pedersen, Ninna, et al.
Publicado: (2014)

Optically Stimulated Luminescent Response of the LiMgPO(4) Silicone Foils to Protons and Its Dependence on Proton Energy
por: Sądel, Michał, et al.
Publicado: (2023)

Validation of miRNA genes suitable as reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar)
por: Johansen, Ilona, et al.
Publicado: (2014)

Application of LiMgPO(4) crystal for proton beam quality control in radiotherapy
por: Wróbel, Damian, et al.
Publicado: (2023)

Relative biological effectiveness of the 60-MeV therapeutic proton beam at the Institute of Nuclear Physics (IFJ PAN) in Kraków, Poland
por: Słonina, Dorota, et al.
Publicado: (2014)

NORMA-Gene: A simple and robust method for qPCR normalization based on target gene data
por: Heckmann, Lars-Henrik, et al.
Publicado: (2011)

In vivo Identification and Specificity assessment of mRNA markers of hypoxia in human and mouse tumors
por: Busk, Morten, et al.
Publicado: (2011)

Modeling RBE with other quantities than LET significantly improves prediction of in vitro cell survival for proton therapy
por: Kalholm, Fredrik, et al.
Publicado: (2022)

Clinical use and future requirements of relative biological effectiveness: survey among all european proton therapy centres
por: Heuchel, Lena, et al.
Publicado: (2022)

Status Report for Experiment AD-4/ACE Biological Effectiveness of Antiproton Annihilation
por: Holzscheiter, M H, et al.
Publicado: (2009)

A unified censored normal regression model for qPCR differential gene expression analysis
por: Pipelers, Peter, et al.
Publicado: (2017)

Calcitriol and Calcidiol Can Sensitize Melanoma Cells to Low–LET Proton Beam Irradiation
por: Podgorska, Ewa, et al.
Publicado: (2018)

Circulating cell-free DNA as predictor of pathological complete response in locally advanced rectal cancer patients undergoing preoperative chemoradiotherapy
por: Truelsen, Christina Glismand, et al.
Publicado: (2022)

Proton beam irradiation inhibits the migration of melanoma cells
por: Jasińska-Konior, Katarzyna, et al.
Publicado: (2017)

Postmenopausal Osteoporosis reference genes for qPCR expression assays
por: de Lima, Camilla Albertina Dantas, et al.
Publicado: (2019)

Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
por: Köhsler, Martina, et al.
Publicado: (2020)

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi
por: Martín-Pérez, Tania, et al.
Publicado: (2023)

Determination of Butyrate Synthesis Capacity in Gut Microbiota: Quantification of but Gene Abundance by qPCR in Fecal Samples
por: Daskova, Nikola, et al.
Publicado: (2021)

Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts
por: Červenák, Zdenko, et al.
Publicado: (2022)

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
por: Svingen, Terje, et al.
Publicado: (2015)

Selection of reliable reference genes for qPCR studies on chondroprotective action
por: Toegel, Stefan, et al.
Publicado: (2007)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Selection of reference genes for qPCR in hairy root cultures of peanut
por: Condori, Jose, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

The Selection of Reliable Reference Genes for RT-qPCR Analysis of Anisakis simplex Sensu Stricto Gene Expression from Different Developmental Stages
por: Łopieńska-Biernat, Elżbieta, et al.
Publicado: (2020)

Reference gene selection for molecular studies of dormancy in wild oat (Avena fatua L.) caryopses by RT-qPCR method
por: Ruduś, Izabela, et al.
Publicado: (2018)

Screening Reliable Reference Genes for RT-qPCR Analysis of Gene Expression in Moringa oleifera
por: Deng, Li-Ting, et al.
Publicado: (2016)

Selection and Validation of Candidate Reference Genes for Gene Expression Analysis by RT-qPCR in Rubus
por: Wu, Yaqiong, et al.
Publicado: (2021)

Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo
por: Hildyard, John C.W., et al.
Publicado: (2022)

Selection of Reference Genes for qPCR- and ddPCR-Based Analyses of Gene Expression in Senescing Barley Leaves
por: Zmienko, Agnieszka, et al.
Publicado: (2015)

Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012)

Pre-amplification in the context of high-throughput qPCR gene expression experiment
por: Korenková, Vlasta, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_h0q43pi016j4kro0ejk8cvbusb